Independent Segregation of Two Antigenic Specificities (VP3 and VP7) Involved in Neutralization of Rotavirus Infectivity

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Dec 1

PMID 3001716

Citations 167

Authors

Y Hoshino

M M Sereno

K Midthun

J Flores

A Z Kapikian

R M Chanock

Affiliations

Soon will be listed here.

Abstract

Antiserum prepared against the M37 strain of rotavirus, recovered from an asymptomatic newborn infant in Venezuela, neutralized two prototype human rotaviruses that define two separate serotypes: serotype 1 (Wa) and serotype 4 (ST3). Thus, the M37 strain is a naturally occurring intertypic rotavirus. Analysis of reassortant viruses produced during coinfection in vitro indicated that the observed dual serotype specificity of M37 resulted from sharing a related outer capsid protein, VP3, with the ST3 virus and another related outer capsid protein, VP7, with the Wa virus. Analysis of single (VP3)-gene-substitution reassortants indicated that VP3 was as potent an immunogen as VP7. In addition, direct evidence was obtained that the serotype specificity of neutralizing antibody elicited by VP3 can differ from the serotype specificity of neutralizing antibody elicited by VP7, indicating the need for a dual system of rotavirus classification in which the neutralization specificity of both VP3 and VP7 outer capsid proteins are identified.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Taniguchi K, URASAWA S, Urasawa T . Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985; 66 ( Pt 5):1045-53. DOI: 10.1099/0022-1317-66-5-1045. View

Kalica A, Greenberg H, Wyatt R, Flores J, Sereno M, Kapikian A . Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981; 112(2):385-90. DOI: 10.1016/0042-6822(81)90285-3. View

Hoshino Y, Wyatt R, Scott F, Appel M . Isolation and characterization of a canine rotavirus. Arch Virol. 1982; 72(1-2):113-25. PMC: 7087106. DOI: 10.1007/BF01314456. View

Wyatt R, Greenberg H, James W, Pittman A, Kalica A, Flores J . Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982; 37(1):110-5. PMC: 347497. DOI: 10.1128/iai.37.1.110-115.1982. View

Flores J, Greenberg H, Myslinski J, Kalica A, Wyatt R, Kapikian A . Use of transcription probes for genotyping rotavirus reassortants. Virology. 1982; 121(2):288-95. DOI: 10.1016/0042-6822(82)90168-4. View

Greenberg H, McAULIFFE V, Valdesuso J, Wyatt R, Flores J, Kalica A . Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983; 39(1):91-9. PMC: 347909. DOI: 10.1128/iai.39.1.91-99.1983. View

Kalica A, Flores J, Greenberg H . Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology. 1983; 125(1):194-205. DOI: 10.1016/0042-6822(83)90073-9. View

Greenberg H, Flores J, Kalica A, Wyatt R, Jones R . Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983; 64 (Pt 2):313-20. DOI: 10.1099/0022-1317-64-2-313. View

10.

Sonza S, Breschkin A, HOLMES I . Derivation of neutralizing monoclonal antibodies against rotavirus. J Virol. 1983; 45(3):1143-6. PMC: 256522. DOI: 10.1128/JVI.45.3.1143-1146.1983. View

11.

Greenberg H, Valdesuso J, Van Wyke K, Midthun K, Walsh M, McAULIFFE V . Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol. 1983; 47(2):267-75. PMC: 255258. DOI: 10.1128/JVI.47.2.267-275.1983. View

12.

Estes M, Palmer E, Obijeski J . Rotaviruses: a review. Curr Top Microbiol Immunol. 1983; 105:123-84. DOI: 10.1007/978-3-642-69159-1_3. View

13.

Hoshino Y, Wyatt R, Greenberg H, Flores J, Kapikian A . Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984; 149(5):694-702. DOI: 10.1093/infdis/149.5.694. View

14.

Bridger J, Brown J . Antigenic and pathogenic relationships of three bovine rotaviruses and a porcine rotavirus. J Gen Virol. 1984; 65 ( Pt 7):1151-8. DOI: 10.1099/0022-1317-65-7-1151. View

15.

Midthun K, Greenberg H, Hoshino Y, Kapikian A, Wyatt R, Chanock R . Reassortant rotaviruses as potential live rotavirus vaccine candidates. J Virol. 1985; 53(3):949-54. PMC: 254731. DOI: 10.1128/JVI.53.3.949-954.1985. View

16.

Coulson B, Fowler K, Bishop R, Cotton R . Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates. J Virol. 1985; 54(1):14-20. PMC: 254754. DOI: 10.1128/JVI.54.1.14-20.1985. View

17.

Hoshino Y, Wyatt R, Flores J, Midthun K, Kapikian A . Serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections. J Clin Microbiol. 1985; 21(3):425-30. PMC: 271678. DOI: 10.1128/jcm.21.3.425-430.1985. View

18.

Greenberg H, Kalica A, Wyatt R, Jones R, Kapikian A, Chanock R . Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proc Natl Acad Sci U S A. 1981; 78(1):420-4. PMC: 319065. DOI: 10.1073/pnas.78.1.420. View