Evaluation of Total Flavonoids, Myricetin, and Quercetin from Hovenia Dulcis Thunb. As Inhibitors of α-Amylase and α-Glucosidase

Overview

Journal Plant Foods Hum Nutr

Specialties Biology
Nutritional Sciences

Date 2016 Oct 28

PMID 27787697

Citations 45

Authors

Yonghong Meng

Anping Su

Shuang Yuan

Huaguo Zhao

Siyuan Tan

Chingyuan Hu

Hong Deng

Yurong Guo

Affiliations

Soon will be listed here.

Abstract

This study was designed to investigate the inhibition effect and mechanism of total flavonoids, myricetin and quercetin extracted from Hovenia dulcis Thunb. on α-amylase and α-glucosidase in order to explore the potential use of Hovenia flavonoids in alleviating postprandial hyperglycemia. The results demonstrate that total flavonoids, myricetin, and quercetin were effective inhibitors of α-amylase with IC values of 32.8, 662 and 770 μg ml, respectively. And all three were effective inhibitors of α-glucosidase with IC values of 8, 3 and 32 μg ml, respectively. Enzyme kinetics tests and Lineweaver-Burk results showed the inhibition effects of total flavonoids, myricetin and quercrtin on α-amylase were all reversible and competitive, and the effects on α-glucosidase were all reversible but non-competitive. This study revealed that Hovenia flavonoids, especially myricetin, are effective and promising functional foods in alleviating type 2 diabetes mellitus.

Citing Articles

Black Gram ( L.) Husk as a Source of Bioactive Compounds: An Evaluation of Starch Digestive Enzyme Inhibition Effects.

Ngoc Dinh C, Prabsangob N Foods. 2025; 14(5).

PMID: 40077549 PMC: 11899647. DOI: 10.3390/foods14050846.

Ultrasound-Assisted Extraction of Polysaccharides from : Structural Analysis and Bioactivity Assessment.

Wu Q, Liang B, Wang J, Dai Y Molecules. 2025; 30(4).

PMID: 40005271 PMC: 11858794. DOI: 10.3390/molecules30040961.

Role of Quercetin in Diabetic Cardiomyopathy.

Mustafa N, Siti H, Kamisah Y Plants (Basel). 2025; 14(1.

PMID: 39795285 PMC: 11722747. DOI: 10.3390/plants14010025.

The Heating Under Micro Variable Pressure (HUMVP) Process to Decrease the Level of Saponin in Quinoa: Evidence of the Antioxidation and the Inhibitory Activity of α-Amylase and α-Glucosidase.

Wu L, Wang A Foods. 2024; 13(22).

PMID: 39594018 PMC: 11593981. DOI: 10.3390/foods13223602.

Suppression of the postprandial hyperglycemia in patients with type 2 diabetes by a raw medicinal herb powder is weakened when consumed in ordinary hard gelatin capsules: A randomized crossover clinical trial.

Moreira F, Reis C, Gallassi A, Moreira D, Welker A PLoS One. 2024; 19(10):e0311501.

PMID: 39383145 PMC: 11463819. DOI: 10.1371/journal.pone.0311501.

References

Guo J, Meng Y, Zhao Y, Hu Y, Ren D, Yang X . Myricetin derived from Hovenia dulcis Thunb. ameliorates vascular endothelial dysfunction and liver injury in high choline-fed mice. Food Funct. 2015; 6(5):1620-34. DOI: 10.1039/c4fo01073f. View

Muller C, Dimitrov Y, Imhoff O, Richter S, Ott J, Krummel T . Oral antidiabetics use among diabetic type 2 patients with chronic kidney disease. Do nephrologists take account of recommendations?. J Diabetes Complications. 2016; 30(4):675-80. DOI: 10.1016/j.jdiacomp.2016.01.016. View

Xie J, Dong C, Nie S, Li F, Wang Z, Shen M . Extraction, chemical composition and antioxidant activity of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja leaves. Food Chem. 2015; 186:97-105. DOI: 10.1016/j.foodchem.2014.06.106. View

Park J, Kim I, Rehman S, Na C, Hyun Yoo H . HPLC Determination of Bioactive Flavonoids in Hovenia dulcis Fruit Extracts. J Chromatogr Sci. 2015; 54(2):130-5. DOI: 10.1093/chromsci/bmv114. View

Peng X, Zhang G, Zeng L . Inhibition of α-glucosidase by vitamin D3 and the effect of vitamins B1 and B2. Food Funct. 2016; 7(2):982-91. DOI: 10.1039/c5fo00992h. View

Wang M, Jiang C, Ma L, Zhang Z, Cao L, Liu J . Preparation, preliminary characterization and immunostimulatory activity of polysaccharide fractions from the peduncles of Hovenia dulcis. Food Chem. 2012; 138(1):41-7. DOI: 10.1016/j.foodchem.2012.09.098. View

Kwon Y, Vattem D, Shetty K . Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr. 2006; 15(1):107-18. View

Park J, Moon J, Park S, Park W, Kim H, Kim J . Fruits extracts of Hovenia dulcis Thunb. suppresses lipopolysaccharide-stimulated inflammatory responses through nuclear factor-kappaB pathway in Raw 264.7 cells. Asian Pac J Trop Med. 2016; 9(4):357-365. DOI: 10.1016/j.apjtm.2016.03.017. View

Wilke T, Boettger B, Berg B, Groth A, Mueller S, Botteman M . Epidemiology of urinary tract infections in type 2 diabetes mellitus patients: An analysis based on a large sample of 456,586 German T2DM patients. J Diabetes Complications. 2015; 29(8):1015-23. DOI: 10.1016/j.jdiacomp.2015.08.021. View

10.

Lin C, Lin C, Yin M . Renal protective effects of extracts from guava fruit (Psidium guajava L.) in diabetic mice. Plant Foods Hum Nutr. 2012; 67(3):303-8. DOI: 10.1007/s11130-012-0294-0. View

11.

Hernandez-Saavedra D, Mendoza-Sanchez M, Hernandez-Montiel H, Guzman-Maldonado H, Loarca-Pina G, Salgado L . Cooked common beans (Phaseolus vulgaris) protect against β-cell damage in streptozotocin-induced diabetic rats. Plant Foods Hum Nutr. 2013; 68(2):207-12. DOI: 10.1007/s11130-013-0353-1. View

12.

Deng Y, Lin-Shiau S, Shyur L, Lin J . Pu-erh tea polysaccharides decrease blood sugar by inhibition of α-glucosidase activity in vitro and in mice. Food Funct. 2015; 6(5):1539-46. DOI: 10.1039/c4fo01025f. View

13.

Dalar A, Turker M, Zabaras D, Konczak I . Phenolic composition, antioxidant and enzyme inhibitory activities of Eryngium bornmuelleri leaf. Plant Foods Hum Nutr. 2013; 69(1):30-6. DOI: 10.1007/s11130-013-0393-6. View