Micro- and Nanoscale Technologies for Delivery into Adherent Cells

Overview

Journal Trends Biotechnol

Specialty Biochemistry

Date 2016 Jun 12

PMID 27287927

Citations 14

Authors

Wonmo Kang

Rebecca L McNaughton

Horacio D Espinosa

Affiliations

Soon will be listed here.

Abstract

Several recent micro- and nanotechnologies have provided novel methods for biological studies of adherent cells because the small features of these new biotools provide unique capabilities for accessing cells without the need for suspension or lysis. These novel approaches have enabled gentle but effective delivery of molecules into specific adhered target cells, with unprecedented spatial resolution. We review here recent progress in the development of these technologies with an emphasis on in vitro delivery into adherent cells utilizing mechanical penetration or electroporation. We discuss the major advantages and limitations of these approaches and propose possible strategies for improvements. Finally, we discuss the impact of these technologies on biological research concerning cell-specific temporal studies, for example non-destructive sampling and analysis of intracellular molecules.

Citing Articles

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Mukherjee P, Peng C, McGuire T, Hwang J, Puritz C, Pathak N Mater Today Bio. 2023; 19:100601.

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Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering.

Patino C, Pathak N, Mukherjee P, Park S, Bao G, Espinosa H Sci Adv. 2022; 8(29):eabn7637.

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Deep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.

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