A Subcellular Cookie Cutter for Spatial Genomics in Human Tissue

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2022 Mar 2

PMID 35233697

Authors

Alexander G Bury

Angela Pyle

Fabio Marcuccio

Doug M Turnbull

Amy E Vincent

Gavin Hudson

Paolo Actis

Affiliations

Soon will be listed here.

Abstract

Intracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease, it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here, we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state-of-the-art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of >20 µm for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.

Citing Articles

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