Single Cell Transcriptomics Reveals Reduced Stress Response in Stem Cells Manipulated Using Localized Electric Fields

Overview

Journal Mater Today Bio

Specialty Biomedical Engineering

Date 2023 Apr 17

PMID 37063248

Authors

Prithvijit Mukherjee

Chian-Yu Peng

Tammy McGuire

Jin Wook Hwang

Connor H Puritz

Nibir Pathak

Cesar A Patino

Rosemary Braun

John A Kessler

Horacio D Espinosa

Affiliations

Soon will be listed here.

Abstract

Membrane disruption using Bulk Electroporation (BEP) is a widely used non-viral method for delivering biomolecules into cells. Recently, its microfluidic counterpart, Localized Electroporation (LEP), has been successfully used for several applications ranging from for therapeutic purposes to from live cells for temporal analysis. However, the side effects of these processes on gene expression, that can affect the physiology of sensitive stem cells are not well understood. Here, we use single cell RNA sequencing (scRNA-seq) to investigate the effects of BEP and LEP on murine neural stem cell (NSC) gene expression. Our results indicate that unlike BEP, LEP does not lead to extensive cell death or activation of cell stress response pathways that may affect their long-term physiology. Additionally, our demonstrations show that LEP is suitable for multi-day delivery protocols as it enables better preservation of cell viability and integrity as compared to BEP.

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