Three Dimensional Time-gated Tracking of Non-blinking Quantum Dots in Live Cells

Overview

Journal Proc SPIE Int Soc Opt Eng

Date 2015 May 2

PMID 25932286

Citations 2

Authors

Matthew S DeVore

Dominik G Stich

Aaron M Keller

Yagnaseni Ghosh

Peter M Goodwin

Mary E Phipps

Michael H Stewart

Cedric Cleyrat

Bridget S Wilson

Diane S Lidke

Jennifer A Hollingsworth

James H Werner

Affiliations

Soon will be listed here.

Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

Citing Articles

Recent Advances in Single-Molecule Tracking and Imaging Techniques.

Nguyen T, Chen Y, Chen L, Yeh H Annu Rev Anal Chem (Palo Alto Calif). 2023; 16(1):253-284.

PMID: 37314878 PMC: 11729782. DOI: 10.1146/annurev-anchem-091922-073057.

Single-Molecule Tracking and Its Application in Biomolecular Binding Detection.

Liu C, Liu Y, Perillo E, Dunn A, Yeh H IEEE J Sel Top Quantum Electron. 2016; 22(4).

PMID: 27660404 PMC: 5028128. DOI: 10.1109/JSTQE.2016.2568160.

Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging.

DeVore M, Stich D, Keller A, Cleyrat C, Phipps M, Hollingsworth J Rev Sci Instrum. 2016; 86(12):126102.

PMID: 26724083 PMC: 4676784. DOI: 10.1063/1.4937477.

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