Confocal, Three-dimensional Tracking of Individual Quantum Dots in High-background Environments

Overview

Journal Anal Chem

Specialty Chemistry

Date 2008 Dec 17

PMID 19072277

Citations 28

Authors

Nathan P Wells

Guillaume A Lessard

James H Werner

Affiliations

Soon will be listed here.

Abstract

We demonstrate a custom confocal fluorescence-microscope that is capable of tracking individual quantum dots undergoing three-dimensional Brownian motion (diffusion coefficient approximately 0.5 microm(2)/s) in environments with a signal-to-background ratio as low as 2:1, significantly worse than observed in a typical cellular environment. By utilizing a pulsed excitation source and time-correlated single photon counting, the time-resolved photon stream can be used to determine changes in the emission lifetime as a function of position and positively identify single quantum dots via photon-pair correlations. These results indicate that this microscope will be capable of following protein and RNA transport throughout the full three-dimensional volume of a live cell for durations up to 15 s.

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