Quantitative Gene Profiling of Long Noncoding RNAs with Targeted RNA Sequencing

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2015 Mar 10

PMID 25751143

Citations 99

Authors

Michael B Clark

Tim R Mercer

Giovanni Bussotti

Tommaso Leonardi

Katelin R Haynes

Joanna Crawford

Marion E Brunck

Kim-Anh Le Cao

Gethin P Thomas

Wendy Y Chen

Ryan J Taft

Lars K Nielsen

Anton J Enright

John S Mattick

Marcel E Dinger

Affiliations

Soon will be listed here.

Abstract

We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.

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