Complete Architecture of the Archaeal RNA Polymerase Open Complex from Single-molecule FRET and NPS

Overview

Journal Nat Commun

Specialty Biology

Date 2015 Jan 31

PMID 25635909

Citations 25

Authors

Julia Nagy

Dina Grohmann

Alan C M Cheung

Sarah Schulz

Katherine Smollett

Finn Werner

Jens Michaelis

Affiliations

Soon will be listed here.

Abstract

The molecular architecture of RNAP II-like transcription initiation complexes remains opaque due to its conformational flexibility and size. Here we report the three-dimensional architecture of the complete open complex (OC) composed of the promoter DNA, TATA box-binding protein (TBP), transcription factor B (TFB), transcription factor E (TFE) and the 12-subunit RNA polymerase (RNAP) from Methanocaldococcus jannaschii. By combining single-molecule Förster resonance energy transfer and the Bayesian parameter estimation-based Nano-Positioning System analysis, we model the entire archaeal OC, which elucidates the path of the non-template DNA (ntDNA) strand and interaction sites of the transcription factors with the RNAP. Compared with models of the eukaryotic OC, the TATA DNA region with TBP and TFB is positioned closer to the surface of the RNAP, likely providing the mechanism by which DNA melting can occur in a minimal factor configuration, without the dedicated translocase/helicase encoding factor TFIIH.

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