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ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide

Overview
Journal Curr Protoc Mol Biol
Specialty Molecular Biology
Date 2015 Jan 7
PMID 25559105
Citations 1638
Authors
Jason D Buenrostro
Beijing Wu
Howard Y Chang
William J Greenleaf
Affiliations
Soon will be listed here.
Abstract

This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription-factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assaying nucleosome positions in accessible regions of the genome.

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