Murine V Kappa Gene Expression Does Not Follow the VH Paradigm

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1989 May 1

PMID 2497227

Citations 20

Authors

A Kaushik

D H Schulze

C Bona

G Kelsoe

Affiliations

Soon will be listed here.

Abstract

V kappa gene family expression among LPS-reactive murine B lymphocytes, unlike that of VH gene families, is not proportional to genomic complexity, i.e., nonstoichiometric. Furthermore, no positional bias for the overexpression of J-proximal V kappa genes (V kappa 21) is observed among neonatal B lymphocytes. Yet, the V kappa 1 and V kappa 9 families located in the center of V kappa locus are preferentially used by neonatal B splenocytes. Thus, the mechanisms of V kappa gene rearrangement and expression appear to differ significantly from those controlling the VH locus.

Citing Articles

Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species.

Sinkora M, Stepanova K, Butler J, Sinkora Jr M, Sinkora S, Sinkorova J Front Immunol. 2022; 13:823145.

PMID: 35222402 PMC: 8873125. DOI: 10.3389/fimmu.2022.823145.

A murine Ig light chain transgene reveals IGKV3 gene contributions to anti-collagen types IV and II specificities.

Clark A, Worni-Schudel I, Korte F, Foster M Mol Immunol. 2017; 91:49-56.

PMID: 28886586 PMC: 5653398. DOI: 10.1016/j.molimm.2017.08.015.

Multiple factors influence the contribution of individual immunoglobulin light chain genes to the naïve antibody repertoire.

Fitzsimmons S, Aydanian A, Clark K, Shapiro M BMC Immunol. 2014; 15:51.

PMID: 25359572 PMC: 4216371. DOI: 10.1186/s12865-014-0051-2.

Skewed primary Igκ repertoire and V-J joining in C57BL/6 mice: implications for recombination accessibility and receptor editing.

Aoki-Ota M, Torkamani A, Ota T, Schork N, Nemazee D J Immunol. 2012; 188(5):2305-15.

PMID: 22287713 PMC: 3288532. DOI: 10.4049/jimmunol.1103484.

Assembly and analysis of the mouse immunoglobulin kappa gene sequence.

Brekke K, Garrard W Immunogenetics. 2004; 56(7):490-505.

PMID: 15378297 DOI: 10.1007/s00251-004-0659-0.

References

Perlmutter R, Kearney J, Chang S, HOOD L . Developmentally controlled expression of immunoglobulin VH genes. Science. 1985; 227(4694):1597-601. DOI: 10.1126/science.3975629. View

Winter E, Radbruch A, Krawinkel U . Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes. EMBO J. 1985; 4(11):2861-7. PMC: 554590. DOI: 10.1002/j.1460-2075.1985.tb04015.x. View

Dildrop R, Krawinkel U, Winter E, Rajewsky K . VH-gene expression in murine lipopolysaccharide blasts distributes over the nine known VH-gene groups and may be random. Eur J Immunol. 1985; 15(11):1154-6. DOI: 10.1002/eji.1830151117. View

Shlomchik M, Nemazee D, Sato V, Van Snick J, Carson D, WEIGERT M . Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). A structural explanation for the high frequency of IgM anti-IgG B cells. J Exp Med. 1986; 164(2):407-27. PMC: 2188225. DOI: 10.1084/jem.164.2.407. View

Schulze D, Kelsoe G . Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice. J Exp Med. 1987; 166(1):163-72. PMC: 2188642. DOI: 10.1084/jem.166.1.163. View

Shapiro M, Weigert M . How immunoglobulin V kappa genes rearrange. J Immunol. 1987; 139(11):3834-9. View

Alt F, Blackwell T, Yancopoulos G . Development of the primary antibody repertoire. Science. 1987; 238(4830):1079-87. DOI: 10.1126/science.3317825. View

Kelsoe G . Cloning of mitogen- and antigen-reactive B lymphocytes on filter paper disks: phenotypic and genotypic analysis of B cell colonies. Methods Enzymol. 1987; 150:287-304. DOI: 10.1016/0076-6879(87)50086-6. View

Jeong H, Komisar J, Kraig E, Teale J . Strain-dependent expression of VH gene families. J Immunol. 1988; 140(7):2436-41. View

10.

Kofler R . A new murine Ig VH gene family. J Immunol. 1988; 140(11):4031-4. View

11.

Reininger L, Kaushik A, Izui S, Jaton J . A member of a new VH gene family encodes antibromelinized mouse red blood cell autoantibodies. Eur J Immunol. 1988; 18(10):1521-6. DOI: 10.1002/eji.1830181008. View

12.

Yancopoulos G, Malynn B, Alt F . Developmentally regulated and strain-specific expression of murine VH gene families. J Exp Med. 1988; 168(1):417-35. PMC: 2188955. DOI: 10.1084/jem.168.1.417. View

13.

DHoostelaere L, Huppi K, Mock B, Mallett C, Potter M . The Ig kappa L chain allelic groups among the Ig kappa haplotypes and Ig kappa crossover populations suggest a gene order. J Immunol. 1988; 141(2):652-61. View

14.

Kasturi K, Monestier M, Mayer R, Bona C . Biased usage of certain Vk gene families by autoantibodies and their polymorphism in autoimmune mice. Mol Immunol. 1988; 25(2):213-9. DOI: 10.1016/0161-5890(88)90070-3. View

15.

Sherwin W, Rowlands Jr D . Determinants of the hierarchy of humoral immune responsiveness during ontogeny. J Immunol. 1975; 115(6):1549-54. View

16.

Gough N, Webb E, Cory S, Adams J . Molecular cloning of seven mouse immunoglobulin kappa chain messenger ribonucleic acids. Biochemistry. 1980; 19(12):2702-10. DOI: 10.1021/bi00553a026. View

17.

Cory S, Tyler B, Adams J . Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes. J Mol Appl Genet. 1981; 1(2):103-16. View

18.

Potter M, Newell J, Rudikoff S, Haber E . Classification of mouse VK groups based on the partial amino acid sequence to the first invariant tryptophan: impact of 14 new sequences from IgG myeloma proteins. Mol Immunol. 1982; 19(12):1619-30. DOI: 10.1016/0161-5890(82)90273-5. View

19.

Gibson D . Evidence for 65 electrophoretically distinct groups of light chains in BALB/c and NZB myelomas. Mol Immunol. 1984; 21(5):421-32. DOI: 10.1016/0161-5890(84)90040-3. View

20.

Brodeur P, Riblet R . The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur J Immunol. 1984; 14(10):922-30. DOI: 10.1002/eji.1830141012. View