The ADP-ribosyltransferase PARP10/ARTD10 Interacts with Proliferating Cell Nuclear Antigen (PCNA) and is Required for DNA Damage Tolerance

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2014 Apr 4

PMID 24695737

Citations 64

Authors

Claudia M Nicolae

Erin R Aho

Alexander H S Vlahos

Katherine N Choe

Subhajyoti De

Georgios I Karras

George-Lucian Moldovan

Affiliations

Soon will be listed here.

Abstract

All cells rely on genomic stability mechanisms to protect against DNA alterations. PCNA is a master regulator of DNA replication and S-phase-coupled repair. PCNA post-translational modifications by ubiquitination and SUMOylation dictate how cells stabilize and re-start replication forks stalled at sites of damaged DNA. PCNA mono-ubiquitination recruits low fidelity DNA polymerases to promote error-prone replication across DNA lesions. Here, we identify the mono-ADP-ribosyltransferase PARP10/ARTD10 as a novel PCNA binding partner. PARP10 knockdown results in genomic instability and DNA damage hypersensitivity. Importantly, we show that PARP10 binding to PCNA is required for translesion DNA synthesis. Our work identifies a novel PCNA-linked mechanism for genome protection, centered on post-translational modification by mono-ADP-ribosylation.

Citing Articles

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PARP enzymes and mono-ADP-ribosylation: advancing the connection from interferon-signalling to cancer biology.

Morone B, Grimaldi G Expert Rev Mol Med. 2024; 26:e17.

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Feijs-Zaja K, Ikenga N, Zaja R Biol Chem. 2024; .

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PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis.

Khatib J, Dhoonmoon A, Moldovan G, Nicolae C Nat Commun. 2024; 15(1):6197.

PMID: 39043663 PMC: 11266678. DOI: 10.1038/s41467-024-50429-3.

K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors.

Waltho A, Popp O, Lenz C, Pluska L, Lambert M, Dotsch V Life Sci Alliance. 2024; 7(8).

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