MiR-210 is Induced by Oct-2, Regulates B Cells, and Inhibits Autoantibody Production

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 2013 Aug 21

PMID 23960236

Citations 30

Authors

Yingting Mok

Vera Schwierzeck

David C Thomas

Elena Vigorito

Tim F Rayner

Lorna B Jarvis

Haydn M Prosser

Allan Bradley

David R Withers

Inga-Lill Martensson

Lynn M Corcoran

Cherie Blenkiron

Eric A Miska

Paul A Lyons

Kenneth G C Smith

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (MiRs) are small, noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we show that MiR-210 is induced by Oct-2, a key transcriptional mediator of B cell activation. Germline deletion of MiR-210 results in the development of autoantibodies from 5 mo of age. Overexpression of MiR-210 in vivo resulted in cell autonomous expansion of the B1 lineage and impaired fitness of B2 cells. Mice overexpressing MiR-210 exhibited impaired class-switched Ab responses, a finding confirmed in wild-type B cells transfected with a MiR-210 mimic. In vitro studies demonstrated defects in cellular proliferation and cell cycle entry, which were consistent with the transcriptomic analysis demonstrating downregulation of genes involved in cellular proliferation and B cell activation. These findings indicate that Oct-2 induction of MiR-210 provides a novel inhibitory mechanism for the control of B cells and autoantibody production.

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