MicroRNA-155 Regulates the Generation of Immunoglobulin Class-switched Plasma Cells

Overview

Journal Immunity

Publisher Cell Press

Specialty Allergy & Immunology

Date 2007 Dec 7

PMID 18055230

Citations 401

Authors

Elena Vigorito

Kerry L Perks

Cei Abreu-Goodger

Sam Bunting

Zou Xiang

Susan Kohlhaas

Partha P Das

Eric A Miska

Antony Rodriguez

Allan Bradley

Kenneth G C Smith

Cristina Rada

Anton J Enright

Kai-Michael Toellner

Ian C M MacLennan

Martin Turner

Affiliations

Soon will be listed here.

Abstract

microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.

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