Resolution Below the Point Spread Function for Diffuse Optical Imaging Using Fluorescence Lifetime Multiplexing

Overview

Journal Opt Lett

Specialty Ophthalmology

Date 2013 Aug 14

PMID 23938969

Citations 5

Authors

William L Rice

Steven Hou

Anand T N Kumar

Affiliations

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Abstract

We show that asymptotic lifetime-based fluorescence tomography can localize multiple-lifetime targets separated well below the diffuse point spread function of a turbid medium. This is made possible due to a complete diagonalization of the time domain forward problem in the asymptotic limit. We also show that continuous wave or direct time gate approaches to fluorescence tomography are unable to achieve this separation, indicating the unique advantage of a decay-amplitude-based approach for tomographic lifetime multiplexing with time domain data.

Citing Articles

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Preclinical whole body time domain fluorescence lifetime multiplexing of fluorescent proteins.

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