Preclinical Whole Body Time Domain Fluorescence Lifetime Multiplexing of Fluorescent Proteins

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2014 Apr 10

PMID 24715027

Citations 6

Authors

William L Rice

Anand T N Kumar

Affiliations

Soon will be listed here.

Abstract

The application of time domain (TD) fluorescence lifetime multiplexing for the detection of fluorescent proteins (FPs) in whole animals, in the presence of a strong background tissue autofluorescence and excitation light leakage is discussed. Tissue autofluorescence (AF) exhibits a nonexponential temporal response, distinct from the mono-exponential decay of FPs. This allows a direct separation of FP fluorescence from AF using a dual basis function approach. We establish the detection limits of this approach using in vitro and in vivo measurements. We also demonstrate, using an experimental model of lymph node metastasis, that FP-AF lifetime multiplexing provides a greater than 30-fold improvement in contrast-to-background ratio compared with continuous wave data. In addition, we show that TD detection can simultaneously discriminate between up to three red shifted FPs placed under the skin of a nude mouse based on their distinct fluorescence lifetimes.

Citing Articles

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