Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages Under Homeostasis

Overview

Journal Immunity

Publisher Cell Press

Specialty Allergy & Immunology

Date 2013 Jan 1

PMID 23273845

Citations 1645

Authors

Simon Yona

Ki-Wook Kim

Yochai Wolf

Alexander Mildner

Diana Varol

Michal Breker

Dalit Strauss-Ayali

Sergey Viukov

Martin Guilliams

Alexander Misharin

David A Hume

Harris Perlman

Bernard Malissen

Elazar Zelzer

Steffen Jung

Affiliations

Soon will be listed here.

Abstract

Mononuclear phagocytes, including monocytes, macrophages, and dendritic cells, contribute to tissue integrity as well as to innate and adaptive immune defense. Emerging evidence for labor division indicates that manipulation of these cells could bear therapeutic potential. However, specific ontogenies of individual populations and the overall functional organization of this cellular network are not well defined. Here we report a fate-mapping study of the murine monocyte and macrophage compartment taking advantage of constitutive and conditional CX(3)CR1 promoter-driven Cre recombinase expression. We have demonstrated that major tissue-resident macrophage populations, including liver Kupffer cells and lung alveolar, splenic, and peritoneal macrophages, are established prior to birth and maintain themselves subsequently during adulthood independent of replenishment by blood monocytes. Furthermore, we have established that short-lived Ly6C(+) monocytes constitute obligatory steady-state precursors of blood-resident Ly6C(-) cells and that the abundance of Ly6C(+) blood monocytes dynamically controls the circulation lifespan of their progeny.

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