Vam3, a Derivative of Resveratrol, Attenuates Cigarette Smoke-induced Autophagy

Overview

Journal Acta Pharmacol Sin

Specialty Pharmacology

Date 2012 Jun 19

PMID 22705731

Citations 27

Authors

Ji Shi

Ning Yin

Ling-Ling Xuan

Chun-Suo Yao

Ai-Min Meng

Qi Hou

Affiliations

Soon will be listed here.

Abstract

Aim: To appraise the efficacy of Vam3 (Amurensis H), a dimeric derivative of resveratrol, at inhibiting cigarette smoke-induced autophagy.

Methods: Human bronchial epithelial cells were treated with cigarette smoke condensates, and a chronic obstructive pulmonary disease (COPD) model was established by exposing male BALB/c mice to cigarette smoke. The protein levels of the autophagic marker microtubule-associated protein 1A/1B-light chain 3 (LC3), Sirtuin 1 (Sirt1), and foxhead box O 3a (FoxO3a) were examined using Western blotting and Immunohistochemistry. LC3 punctae were detected by immunofluorescence. The levels of FoxO3a acetylation were examined by immunoprecipitation. The level of intracellular oxidation was assessed by detecting ROS and GSH-Px.

Results: Vam3 attenuated cigarette smoke condensate-induced autophagy in human bronchial epithelial cells, and restored the expression levels of Sirt1 and FoxO3a that had been reduced by cigarette smoke condensates. Similar protective effects of Vam3, reducing autophagy and restoring the levels of Sirt1 and FoxO3a, were observed in the COPD animal model. Additionally, Vam3 also diminished the oxidative stress that was induced by the cigarette smoke condensates.

Conclusion: Vam3 decreases cigarette smoke-induced autophagy via up-regulating/restoring the levels of Sirt1 and FoxO3a and inhibiting the induced oxidative stress.

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References

Wood L, Wark P, Garg M . Antioxidant and anti-inflammatory effects of resveratrol in airway disease. Antioxid Redox Signal. 2010; 13(10):1535-48. DOI: 10.1089/ars.2009.3064. View

Cohen H, Lavu S, Bitterman K, Hekking B, Imahiyerobo T, Miller C . Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis. Mol Cell. 2004; 13(5):627-38. DOI: 10.1016/s1097-2765(04)00094-2. View

Vahtola E, Louhelainen M, Forsten H, Merasto S, Raivio J, Kaheinen P . Sirtuin1-p53, forkhead box O3a, p38 and post-infarct cardiac remodeling in the spontaneously diabetic Goto-Kakizaki rat. Cardiovasc Diabetol. 2010; 9:5. PMC: 2835668. DOI: 10.1186/1475-2840-9-5. View

Lakhdar R, Denden S, Kassab A, Leban N, Knani J, Lefranc G . Update in chronic obstructive pulmonary disease: role of antioxidant and metabolizing gene polymorphisms. Exp Lung Res. 2011; 37(6):364-75. DOI: 10.3109/01902148.2011.580416. View

Klionsky D, Cuervo A, Seglen P . Methods for monitoring autophagy from yeast to human. Autophagy. 2007; 3(3):181-206. DOI: 10.4161/auto.3678. View

Kitamura T, Kitamura Y . [The roles of PPAR, C/EBP and FoxO families in adipocyte differentiation and proliferation]. Nihon Rinsho. 2011; 69 Suppl 1:259-63. View

Yang L, Yao C, Wu Z, Xuan L, Bai J, Cheng G . [Effects of dihydroxy-stilbene compound Vam3 on airway inflammation, expression of ICAM-1, activities of NF-kappaB and MMP-9 in asthmatic mice]. Yao Xue Xue Bao. 2011; 45(12):1503-8. View

Hwang J, Chung S, Sundar I, Yao H, Arunachalam G, McBurney M . Cigarette smoke-induced autophagy is regulated by SIRT1-PARP-1-dependent mechanism: implication in pathogenesis of COPD. Arch Biochem Biophys. 2010; 500(2):203-9. PMC: 2904411. DOI: 10.1016/j.abb.2010.05.013. View

Dansen T, Smits L, van Triest M, de Keizer P, van Leenen D, Koerkamp M . Redox-sensitive cysteines bridge p300/CBP-mediated acetylation and FoxO4 activity. Nat Chem Biol. 2009; 5(9):664-72. DOI: 10.1038/nchembio.194. View

10.

Yuan J, Ma J, Zheng H, Shi T, Sun W, Zhang Q . Overexpression of OLC1, cigarette smoke, and human lung tumorigenesis. J Natl Cancer Inst. 2008; 100(22):1592-605. PMC: 3299210. DOI: 10.1093/jnci/djn379. View

11.

Rajendran R, Garva R, Krstic-Demonacos M, Demonacos C . Sirtuins: molecular traffic lights in the crossroad of oxidative stress, chromatin remodeling, and transcription. J Biomed Biotechnol. 2011; 2011:368276. PMC: 3168296. DOI: 10.1155/2011/368276. View

12.

Chen Z, Kim H, Sciurba F, Lee S, Feghali-Bostwick C, Stolz D . Egr-1 regulates autophagy in cigarette smoke-induced chronic obstructive pulmonary disease. PLoS One. 2008; 3(10):e3316. PMC: 2552992. DOI: 10.1371/journal.pone.0003316. View

13.

Valenti V, de Abreu L, Sato M, Ferreira C, Adami F, Fonseca F . Sidestream cigarette smoke effects on cardiovascular responses in conscious rats: involvement of oxidative stress in the fourth cerebral ventricle. BMC Cardiovasc Disord. 2012; 12:22. PMC: 3352042. DOI: 10.1186/1471-2261-12-22. View

14.

Huang K, Lin M, Cheng G . Anti-inflammatory tetramers of resveratrol from the roots of Vitis amurensis and the conformations of the seven-membered ring in some oligostilbenes. Phytochemistry. 2001; 58(2):357-62. DOI: 10.1016/s0031-9422(01)00224-2. View

15.

Chung S, Yao H, Caito S, Hwang J, Arunachalam G, Rahman I . Regulation of SIRT1 in cellular functions: role of polyphenols. Arch Biochem Biophys. 2010; 501(1):79-90. PMC: 2930135. DOI: 10.1016/j.abb.2010.05.003. View

16.

Bjorkoy G, Lamark T, Pankiv S, Overvatn A, Brech A, Johansen T . Monitoring autophagic degradation of p62/SQSTM1. Methods Enzymol. 2009; 452:181-97. DOI: 10.1016/S0076-6879(08)03612-4. View

17.

Zhang X, Xiao T, Cheng S, Tong T, Gao Y . Cigarette smoke suppresses the ubiquitin-dependent degradation of OLC1. Biochem Biophys Res Commun. 2011; 407(4):753-7. DOI: 10.1016/j.bbrc.2011.03.095. View

18.

Brunet A, Sweeney L, Sturgill J, Chua K, Greer P, Lin Y . Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 2004; 303(5666):2011-5. DOI: 10.1126/science.1094637. View

19.

Zhao Y, Wang Y, Zhu W . Applications of post-translational modifications of FoxO family proteins in biological functions. J Mol Cell Biol. 2011; 3(5):276-82. DOI: 10.1093/jmcb/mjr013. View

20.

Knobloch J, Sibbing B, Jungck D, Lin Y, Urban K, Stoelben E . Resveratrol impairs the release of steroid-resistant inflammatory cytokines from human airway smooth muscle cells in chronic obstructive pulmonary disease. J Pharmacol Exp Ther. 2010; 335(3):788-98. DOI: 10.1124/jpet.110.166843. View