Antigenic and Genetic Analyses of Human Rotavirus with Dual Subgroup Specificity

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1990 Dec 1

PMID 2177756

Citations 6

Authors

T Urasawa

K Taniguchi

N Kobayashi

F WAKASUGI

I Oishi

Y Minekawa

M Oseto

M U Ahmed

S URASAWA

Affiliations

Soon will be listed here.

Abstract

In our previous study (S. Urasawa, T. Urasawa, K. Taniguchi, F. Wakasugi, N. Kobayashi, S. Chiba, N. Sakurada, S. Morita, O. Morita, M. Tokieda, T. Kawamoto, K. Minekawa, and M. Oseto, J. Infect. Dis. 160:44-51, 1989) of antigenic characterization of about 300 human rotavirus (HRV) isolates collected at different localities in Japan, we found 4 HRV isolates having unique antigenic and genetic constructions. The four strains possessed both subgroup I and subgroup II antigens, serotype 3 antigen, and a long RNA electropherotype. The reactivity pattern of these four HRV isolates with three monoclonal antibodies (MAbs) directed to an outer capsid protein, VP4, and with one MAb directed to an inner capsid protein, VP2, was clearly different from those of usual subgroup II HRVs having serotype 1, serotype 3, or serotype 4 specificity and a long RNA pattern, whereas their reactivity pattern was similar to that of strain K8 (subgroup II, serotype 1), which possessed unique VP4 and VP2 proteins. RNA-RNA cross-hybridization analysis indicated that while the four isolates were genetically distinct from the two genetic groups of HRV reported previously, i.e., the Wa family (strains KU, S3, and YO) and the DS-1 family (strain S2), they were closely related to strain K8, a strain having unique antigenic and genetic properties (K. Taniguchi, K. Nishikawa, T. Urasawa, S. Urasawa, K. Midthun, A. Z. Kapikian, and M. Gorziglia, J. Virol. 63:4101-4106, 1989).

Citing Articles

Detection of a human rotavirus with G12 and P[9] specificity in Thailand.

Pongsuwanna Y, Guntapong R, Chiwakul M, Tacharoenmuang R, Onvimala N, Wakuda M J Clin Microbiol. 2002; 40(4):1390-4.

PMID: 11923362 PMC: 140366. DOI: 10.1128/JCM.40.4.1390-1394.2002.

Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by electropherotype, subgroup and serotype analysis.

Aijaz S, Gowda K, Jagannath H, Reddy R, Maiya P, Ward R Arch Virol. 1996; 141(3-4):715-26.

PMID: 8645107 DOI: 10.1007/BF01718329.

Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction.

Wu H, Taniguchi K, WAKASUGI F, Ukae S, Chiba S, Ohseto M Epidemiol Infect. 1994; 112(3):615-22.

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Preparation and characterization of a neutralizing monoclonal antibody directed to VP4 of rotavirus strain K8 which has unique VP4 neutralization epitopes.

Kobayashi N, Taniguchi K, Urasawa T, URASAWA S Arch Virol. 1991; 121(1-4):153-62.

PMID: 1722090 DOI: 10.1007/BF01316751.

Antibody response to serotype-specific and cross-reactive neutralization epitopes on VP4 and VP7 after rotavirus infection or vaccination.

Taniguchi K, Urasawa T, Kobayashi N, Ahmed M, Adachi N, Chiba S J Clin Microbiol. 1991; 29(3):483-7.

PMID: 1709946 PMC: 269805. DOI: 10.1128/jcm.29.3.483-487.1991.

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