The Nuclear-retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor Phosphorylation

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Aug 28

PMID 20797886

Citations 1211

Authors

Vidisha Tripathi

Jonathan D Ellis

Zhen Shen

David Y Song

Qun Pan

Andrew T Watt

Susan M Freier

C Frank Bennett

Alok Sharma

Paula A Bubulya

Benjamin J Blencowe

Supriya G Prasanth

Kannanganattu V Prasanth

Affiliations

Soon will be listed here.

Abstract

Alternative splicing (AS) of pre-mRNA is utilized by higher eukaryotes to achieve increased transcriptome and proteomic complexity. The serine/arginine (SR) splicing factors regulate tissue- or cell-type-specific AS in a concentration- and phosphorylation-dependent manner. However, the mechanisms that modulate the cellular levels of active SR proteins remain to be elucidated. In the present study, we provide evidence for a role for the long nuclear-retained regulatory RNA (nrRNA), MALAT1 in AS regulation. MALAT1 interacts with SR proteins and influences the distribution of these and other splicing factors in nuclear speckle domains. Depletion of MALAT1 or overexpression of an SR protein changes the AS of a similar set of endogenous pre-mRNAs. Furthermore, MALAT1 regulates cellular levels of phosphorylated forms of SR proteins. Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression.

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