Quantitation of the Ribosomal Protein Autoregulatory Network Using Mass Spectrometry

Overview

Journal Anal Chem

Specialty Chemistry

Date 2010 May 21

PMID 20481440

Citations 12

Authors

Michael T Sykes

Edit Sperling

Stephen S Chen

James R Williamson

Affiliations

Soon will be listed here.

Abstract

Relative levels of ribosomal proteins were quantified in crude cell lysates using mass spectrometry. A method for quantifying cellular protein levels using macromolecular standards is presented that does not require complex sample separation, identification of high-responding peptides, affinity purification, or postgrowth modifications. Perturbations in ribosomal protein levels by overexpression of individual proteins correlate to known autoregulatory mechanisms and extend the network of ribosomal protein regulation.

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