Quantitative Interaction Proteomics Using Mass Spectrometry

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2009 Feb 10

PMID 19198594

Citations 52

Authors

Alexander Wepf

Timo Glatter

Alexander Schmidt

Ruedi Aebersold

Matthias Gstaiger

Affiliations

Soon will be listed here.

Abstract

We present a mass spectrometry-based strategy for the absolute quantification of protein complex components isolated through affinity purification. We quantified bait proteins via isotope-labeled reference peptides corresponding to an affinity tag sequence and prey proteins by label-free correlational quantification using the precursor ion signal intensities of proteotypic peptides generated in reciprocal purifications. We used this method to quantitatively analyze interaction stoichiometries in the human protein phosphatase 2A network.

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