MicroRNA-127 Modulates Fetal Lung Development

Overview

Journal Physiol Genomics

Publisher American Physiological Society

Specialties Genetics
Molecular Biology

Date 2009 May 15

PMID 19439715

Citations 86

Authors

Manoj Bhaskaran

Yang Wang

Honghao Zhang

Tingting Weng

Pradyumna Baviskar

Yujie Guo

Deming Gou

Lin Liu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are small endogenous RNAs and are widely regarded as one of the most important regulators of gene expression in both plants and animals. To define the roles of miRNAs in fetal lung development, we profiled the miRNA expression pattern during lung development with a miRNA microarray. We identified 21 miRNAs that showed significant changes in expression during lung development. These miRNAs were grouped into four distinct clusters based on their expression pattern. Cluster 1 contained miRNAs whose expression increased as development progressed, while clusters 2 and 3 showed the opposite trend of expression. miRNAs in cluster 4 including miRNA-127 (miR-127) had the highest expression at the late stage of fetal lung development. Quantitative real-time PCR validated the microarray results of six selected miRNAs. In situ hybridization demonstrated that miR-127 expression gradually shifted from mesenchymal cells to epithelial cells as development progressed. Overexpression of miR-127 in fetal lung organ culture significantly decreased the terminal bud count, increased terminal and internal bud sizes, and caused unevenness in bud sizes, indicating improper development. These findings suggest that miR-127 may have an important role in fetal lung development.

Citing Articles

Influence of Microenvironmental Orchestration on Multicellular Lung Alveolar Organoid Development from Human Induced Pluripotent Stem Cells.

Ozan V, Wang H, Akshay A, Anand D, Hibaoui Y, Feki A Stem Cell Rev Rep. 2024; 21(1):254-275.

PMID: 39417930 PMC: 11762634. DOI: 10.1007/s12015-024-10789-1.

Discovering genes and microRNAs involved in human lung development unveils IGFBP3/miR-34a dynamics and their relevance for alveolar differentiation.

Acosta-Plasencia M, Castellano J, Diaz T, He Y, Marrades R, Navarro A Stem Cell Res Ther. 2024; 15(1):263.

PMID: 39183355 PMC: 11346212. DOI: 10.1186/s13287-024-03883-1.

Amniotic fluid-derived small extracellular vesicles for predicting postnatal severe outcome of congenital diaphragmatic hernia.

Matsuo S, Yokoi A, Yoshida K, Kitagawa M, Asano-Inami E, Miura M J Extracell Biol. 2024; 3(6):e160.

PMID: 38947173 PMC: 11212330. DOI: 10.1002/jex2.160.

The Binding of to Cystic Fibrosis Bronchial Epithelial Model Cells Alters the Composition of the Exosomes They Produce Compared to Healthy Control Cells.

Lozano-Iturbe V, Blanco-Agudin N, Vazquez-Espinosa E, Fernandez-Vega I, Merayo-Lloves J, Vazquez F Int J Mol Sci. 2024; 25(2).

PMID: 38255969 PMC: 10815301. DOI: 10.3390/ijms25020895.

TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis.

Wang L, Zhao W, Xia C, Ma S, Li Z, Wang N Cell Mol Life Sci. 2023; 81(1):13.

PMID: 38157020 PMC: 10756874. DOI: 10.1007/s00018-023-05080-4.

References

Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M . Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell. 2006; 9(3):189-98. DOI: 10.1016/j.ccr.2006.01.025. View

He L, Thomson J, Hemann M, Hernando-Monge E, Mu D, Goodson S . A microRNA polycistron as a potential human oncogene. Nature. 2005; 435(7043):828-33. PMC: 4599349. DOI: 10.1038/nature03552. View

Gou D, Narasaraju T, Chintagari N, Jin N, Wang P, Liu L . Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo. Nucleic Acids Res. 2004; 32(17):e134. PMC: 521678. DOI: 10.1093/nar/gnh129. View

Seitz H, Youngson N, Lin S, Dalbert S, Paulsen M, Bachellerie J . Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene. Nat Genet. 2003; 34(3):261-2. DOI: 10.1038/ng1171. View

Filipowicz W, Bhattacharyya S, Sonenberg N . Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?. Nat Rev Genet. 2008; 9(2):102-14. DOI: 10.1038/nrg2290. View

Hatfield S, Shcherbata H, Fischer K, Nakahara K, Carthew R, Ruohola-Baker H . Stem cell division is regulated by the microRNA pathway. Nature. 2005; 435(7044):974-8. DOI: 10.1038/nature03816. View

Vasudevan S, Tong Y, Steitz J . Switching from repression to activation: microRNAs can up-regulate translation. Science. 2007; 318(5858):1931-4. DOI: 10.1126/science.1149460. View

Blenkiron C, Goldstein L, Thorne N, Spiteri I, Chin S, Dunning M . MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype. Genome Biol. 2007; 8(10):R214. PMC: 2246288. DOI: 10.1186/gb-2007-8-10-r214. View

Perry M, Moschos S, Williams A, Shepherd N, Larner-Svensson H, Lindsay M . Rapid changes in microRNA-146a expression negatively regulate the IL-1beta-induced inflammatory response in human lung alveolar epithelial cells. J Immunol. 2008; 180(8):5689-98. PMC: 2639646. DOI: 10.4049/jimmunol.180.8.5689. View

10.

Shcherbata H, Hatfield S, Ward E, Reynolds S, Fischer K, Ruohola-Baker H . The MicroRNA pathway plays a regulatory role in stem cell division. Cell Cycle. 2005; 5(2):172-5. DOI: 10.4161/cc.5.2.2343. View

11.

Williams A, Moschos S, Perry M, Barnes P, Lindsay M . Maternally imprinted microRNAs are differentially expressed during mouse and human lung development. Dev Dyn. 2006; 236(2):572-80. PMC: 2582151. DOI: 10.1002/dvdy.21047. View

12.

Ma L, Teruya-Feldstein J, Weinberg R . Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007; 449(7163):682-8. DOI: 10.1038/nature06174. View

13.

Wu L, Belasco J . Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAs. Mol Cell. 2008; 29(1):1-7. DOI: 10.1016/j.molcel.2007.12.010. View

14.

Johnston R, Hobert O . A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans. Nature. 2003; 426(6968):845-9. DOI: 10.1038/nature02255. View

15.

Xiao C, Calado D, Galler G, Thai T, Patterson H, Wang J . MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007; 131(1):146-59. DOI: 10.1016/j.cell.2007.07.021. View

16.

Burri P . Fetal and postnatal development of the lung. Annu Rev Physiol. 1984; 46:617-28. DOI: 10.1146/annurev.ph.46.030184.003153. View

17.

Chen X . A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science. 2003; 303(5666):2022-5. PMC: 5127708. DOI: 10.1126/science.1088060. View

18.

Lee Y, Jeon K, Lee J, Kim S, Kim V . MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 2002; 21(17):4663-70. PMC: 126204. DOI: 10.1093/emboj/cdf476. View

19.

Lodish H, Zhou B, Liu G, Chen C . Micromanagement of the immune system by microRNAs. Nat Rev Immunol. 2008; 8(2):120-30. DOI: 10.1038/nri2252. View

20.

Moschos S, Williams A, Perry M, Birrell M, Belvisi M, Lindsay M . Expression profiling in vivo demonstrates rapid changes in lung microRNA levels following lipopolysaccharide-induced inflammation but not in the anti-inflammatory action of glucocorticoids. BMC Genomics. 2007; 8:240. PMC: 1940008. DOI: 10.1186/1471-2164-8-240. View