A MicroRNA As a Translational Repressor of APETALA2 in Arabidopsis Flower Development

Overview

Journal Science

Specialty Science

Date 2003 Aug 2

PMID 12893888

Citations 758

Authors

Xuemei Chen

Affiliations

Soon will be listed here.

Abstract

Plant microRNAs (miRNAs) show a high degree of sequence complementarity to, and are believed to guide the cleavage of, their target messenger RNAs. Here, I show that miRNA172, which can base-pair with the messenger RNA of a floral homeotic gene, APETALA2, regulates APETALA2 expression primarily through translational inhibition. Elevated miRNA172 accumulation results in floral organ identity defects similar to those in loss-of-function apetala2 mutants. Elevated levels of mutant APETALA2 RNA with disrupted miRNA172 base pairing, but not wild-type APETALA2 RNA, result in elevated levels of APETALA2 protein and severe floral patterning defects. Therefore, miRNA172 likely acts in cell-fate specification as a translational repressor of APETALA2 in Arabidopsis flower development.

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References

Bowman J, Smyth D, Meyerowitz E . Genetic interactions among floral homeotic genes of Arabidopsis. Development. 1991; 112(1):1-20. DOI: 10.1242/dev.112.1.1. View

Llave C, Xie Z, Kasschau K, Carrington J . Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science. 2002; 297(5589):2053-6. DOI: 10.1126/science.1076311. View

Lohmann J, Weigel D . Building beauty: the genetic control of floral patterning. Dev Cell. 2002; 2(2):135-42. DOI: 10.1016/s1534-5807(02)00122-3. View

Aukerman M, Sakai H . Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell. 2003; 15(11):2730-41. PMC: 280575. DOI: 10.1105/tpc.016238. View

Tang G, Reinhart B, Bartel D, Zamore P . A biochemical framework for RNA silencing in plants. Genes Dev. 2003; 17(1):49-63. PMC: 195971. DOI: 10.1101/gad.1048103. View

Ambros V . MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell. 2003; 113(6):673-6. DOI: 10.1016/s0092-8674(03)00428-8. View

Reinhart B, Slack F, Basson M, Pasquinelli A, Bettinger J, Rougvie A . The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 2000; 403(6772):901-6. DOI: 10.1038/35002607. View

Kasschau K, Xie Z, Allen E, Llave C, Chapman E, Krizan K . P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction. Dev Cell. 2003; 4(2):205-17. DOI: 10.1016/s1534-5807(03)00025-x. View

Xie Z, Kasschau K, Carrington J . Negative feedback regulation of Dicer-Like1 in Arabidopsis by microRNA-guided mRNA degradation. Curr Biol. 2003; 13(9):784-9. DOI: 10.1016/s0960-9822(03)00281-1. View

10.

Pasquinelli A, Ruvkun G . Control of developmental timing by micrornas and their targets. Annu Rev Cell Dev Biol. 2002; 18:495-513. DOI: 10.1146/annurev.cellbio.18.012502.105832. View

11.

Mizukami Y, Ma H . Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992; 71(1):119-31. DOI: 10.1016/0092-8674(92)90271-d. View

12.

Goodrich J, Puangsomlee P, Martin M, Long D, Meyerowitz E, Coupland G . A Polycomb-group gene regulates homeotic gene expression in Arabidopsis. Nature. 1997; 386(6620):44-51. DOI: 10.1038/386044a0. View

13.

Olsen P, Ambros V . The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev Biol. 2000; 216(2):671-80. DOI: 10.1006/dbio.1999.9523. View

14.

Park W, Li J, Song R, Messing J, Chen X . CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. Curr Biol. 2002; 12(17):1484-95. PMC: 5137372. DOI: 10.1016/s0960-9822(02)01017-5. View

15.

Chen X, Meyerowitz E . HUA1 and HUA2 are two members of the floral homeotic AGAMOUS pathway. Mol Cell. 1999; 3(3):349-60. DOI: 10.1016/s1097-2765(00)80462-1. View

16.

Bartel B, Bartel D . MicroRNAs: at the root of plant development?. Plant Physiol. 2003; 132(2):709-17. PMC: 523861. DOI: 10.1104/pp.103.023630. View

17.

Chen X, Liu J, Cheng Y, Jia D . HEN1 functions pleiotropically in Arabidopsis development and acts in C function in the flower. Development. 2002; 129(5):1085-94. PMC: 5137379. DOI: 10.1242/dev.129.5.1085. View

18.

Jofuku K, den Boer B, Van Montagu M, Okamuro J . Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell. 1994; 6(9):1211-25. PMC: 160514. DOI: 10.1105/tpc.6.9.1211. View