Long Noncoding RNA MALAT1 Sponges MiR-129-5p to Regulate the Development of Bronchopulmonary Dysplasia by Increasing the Expression of

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2020 May 14

PMID 32397779

Citations 7

Authors

Rongwe Yangi

Huafei Huang

Qingnv Zhou

Affiliations

Soon will be listed here.

Abstract

Objective: To explore the function and mechanism of long noncoding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in bronchopulmonary dysplasia.

Methods: Alveolar epithelial cell line BEAS-2B was used as the cell model. The role of MALAT1 and microRNA miR-129-5p in regulating cellular viability and migration were examined by using the CCK-8 and Transwell assays, respectively, in vitro. The luciferase reporter assay and real-time (RT)-PCR were performed to confirm that miR-129-5p was a target of MALAT1. ELISA was conducted to validate MALAT1 and show that miR-129-5p regulated the gene encoding high-mobility group protein 1 ().

Results: Overexpression of MALAT1 significantly promoted cellular viability, whereas miR-129-5p had the opposite effect. miR-129-5p was shown to be a target of MALAT1, and HMGB1 could be upregulated by MALAT1 overexpression or miR-129-5p inhibition.

Conclusion: MALAT1 reduced the expression of miR-129-5p, promoting the viability of cells and blocking the development of bronchopulmonary dysplasia. In addition, MALAT1 increased the expression of HMGB1, which contributed to inflammation as the disease progressed.

Citing Articles

Circular RNAs expression profiles and bioinformatics analysis in bronchopulmonary dysplasia.

Lun Y, Hu J, Zuming Y J Clin Lab Anal. 2022; 37(1):e24805.

PMID: 36514862 PMC: 9833990. DOI: 10.1002/jcla.24805.

Mechanistic studies of MALAT1 in respiratory diseases.

Wu W, Wang S, Zhang L, Mao B, Wang B, Wang X Front Mol Biosci. 2022; 9:1031861.

PMID: 36419933 PMC: 9676952. DOI: 10.3389/fmolb.2022.1031861.

Focus on long non-coding RNA MALAT1: Insights into acute and chronic lung diseases.

Lai X, Zhong J, Zhang A, Zhang B, Zhu T, Liao R Front Genet. 2022; 13:1003964.

PMID: 36186445 PMC: 9523402. DOI: 10.3389/fgene.2022.1003964.

Insight Into the Roles of Non-coding RNA in Bronchopulmonary Dysplasia.

Xi Y, Wang Y Front Med (Lausanne). 2021; 8:761724.

PMID: 34805228 PMC: 8602187. DOI: 10.3389/fmed.2021.761724.

MALAT1 shuttled by extracellular vesicles promotes M1 polarization of macrophages to induce acute pancreatitis via miR-181a-5p/HMGB1 axis.

Liu J, Niu Z, Zhang R, Peng Z, Wang L, Liu Z J Cell Mol Med. 2021; 25(19):9241-9254.

PMID: 34448533 PMC: 8500974. DOI: 10.1111/jcmm.16844.

References

Luo J, Chen J, He L . mir-129-5p Attenuates Irradiation-Induced Autophagy and Decreases Radioresistance of Breast Cancer Cells by Targeting HMGB1. Med Sci Monit. 2016; 21:4122-9. PMC: 4700864. DOI: 10.12659/msm.896661. View

Jung E, Park K, Han B, Cho S, Yoo H, Lee J . Amniotic Fluid Infection, Cytokine Levels, and Mortality and Adverse Pulmonary, Intestinal, and Neurologic Outcomes in Infants at 32 Weeks' Gestation or Less. J Korean Med Sci. 2017; 32(3):480-487. PMC: 5290108. DOI: 10.3346/jkms.2017.32.3.480. View

Zhang L, Ji Y, Kang Z, Lv C, Jiang W . Protocatechuic aldehyde ameliorates experimental pulmonary fibrosis by modulating HMGB1/RAGE pathway. Toxicol Appl Pharmacol. 2015; 283(1):50-6. DOI: 10.1016/j.taap.2015.01.001. View

Sayed D, Abdellatif M . MicroRNAs in development and disease. Physiol Rev. 2011; 91(3):827-87. DOI: 10.1152/physrev.00006.2010. View

Zhang Y, An J, Lv W, Lou T, Liu Y, Kang W . miRNA-129-5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E-cadherin and vimentin. Oncol Lett. 2017; 12(6):5163-5169. PMC: 5228557. DOI: 10.3892/ol.2016.5372. View

Popova A . Mechanisms of bronchopulmonary dysplasia. J Cell Commun Signal. 2013; 7(2):119-27. PMC: 3660689. DOI: 10.1007/s12079-013-0190-x. View

Tano K, Mizuno R, Okada T, Rakwal R, Shibato J, Masuo Y . MALAT-1 enhances cell motility of lung adenocarcinoma cells by influencing the expression of motility-related genes. FEBS Lett. 2010; 584(22):4575-80. DOI: 10.1016/j.febslet.2010.10.008. View

Ding J, Cui X, Liu Q . Emerging role of HMGB1 in lung diseases: friend or foe. J Cell Mol Med. 2017; 21(6):1046-1057. PMC: 5431121. DOI: 10.1111/jcmm.13048. View

Principi N, Di Pietro G, Esposito S . Bronchopulmonary dysplasia: clinical aspects and preventive and therapeutic strategies. J Transl Med. 2018; 16(1):36. PMC: 5819643. DOI: 10.1186/s12967-018-1417-7. View

10.

Zhang X, Hamblin M, Yin K . The long noncoding RNA Malat1: Its physiological and pathophysiological functions. RNA Biol. 2017; 14(12):1705-1714. PMC: 5731810. DOI: 10.1080/15476286.2017.1358347. View

11.

Iyengar A, Davis J . Drug therapy for the prevention and treatment of bronchopulmonary dysplasia. Front Pharmacol. 2015; 6:12. PMC: 4329808. DOI: 10.3389/fphar.2015.00012. View

12.

Lelli A, Nolan K, Santambrogio S, Goncalves A, Schonenberger M, Guinot A . Induction of long noncoding RNA MALAT1 in hypoxic mice. Hypoxia (Auckl). 2016; 3:45-52. PMC: 5045088. DOI: 10.2147/HP.S90555. View

13.

de Souza A, Chirico M, Cartelle C, Costa G, Talvani A, Cangussu S . High-Fat Diet Increases HMGB1 Expression and Promotes Lung Inflammation in Mice Subjected to Mechanical Ventilation. Oxid Med Cell Longev. 2018; 2018:7457054. PMC: 5830287. DOI: 10.1155/2018/7457054. View

14.

Yang Y, Kai G, Pu X, Qing K, Guo X, Zhou X . Expression profile of microRNAs in fetal lung development of Sprague-Dawley rats. Int J Mol Med. 2011; 29(3):393-402. DOI: 10.3892/ijmm.2011.855. View

15.

Botti G, Marra L, Malzone M, Anniciello A, Botti C, Franco R . LncRNA HOTAIR as Prognostic Circulating Marker and Potential Therapeutic Target in Patients with Tumor Diseases. Curr Drug Targets. 2015; 18(1):27-34. DOI: 10.2174/1389450117666151209122950. View

16.

Wang S, Chen Y, Yu X, Lu Y, Wang H, Wu F . miR-129-5p attenuates cell proliferation and epithelial mesenchymal transition via HMGB1 in gastric cancer. Pathol Res Pract. 2019; 215(4):676-682. DOI: 10.1016/j.prp.2018.12.024. View

17.

Sauvageau M, Goff L, Lodato S, Bonev B, Groff A, Gerhardinger C . Multiple knockout mouse models reveal lincRNAs are required for life and brain development. Elife. 2014; 2:e01749. PMC: 3874104. DOI: 10.7554/eLife.01749. View

18.

Michalik K, You X, Manavski Y, Doddaballapur A, Zornig M, Braun T . Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth. Circ Res. 2014; 114(9):1389-97. DOI: 10.1161/CIRCRESAHA.114.303265. View

19.

Feng J, Deng C, Yu J, Guo C, Zhao Q . [Expression of high mobility group protein-B1 in mice with hyperoxia-induced bronchopulmonary dysplasia]. Zhongguo Dang Dai Er Ke Za Zhi. 2010; 12(3):219-23. View

20.

Liu K, Huang J, Ni J, Song D, Ding M, Wang J . MALAT1 promotes osteosarcoma development by regulation of HMGB1 via miR-142-3p and miR-129-5p. Cell Cycle. 2017; 16(6):578-587. PMC: 5384591. DOI: 10.1080/15384101.2017.1288324. View