Identification and Control of Synthesis of the DsdC Activator Protein

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Feb 1

PMID 6296057

Citations 13

Authors

E MCFALL

M C Heincz

Affiliations

Soon will be listed here.

Abstract

Operon fusions between the D-serine deaminase regulatory and structural genes and lacZ were constructed and used to examine the control of expression of the positive regulatory gene, dsdC. Merodiploid strains containing both dsdCp::Mu d (lac Apr) and dsdC+A+ produced only one-fourth as much beta-galactosidase as did the haploid dsdCp::Mu d (lac Apr) strains, indicating that the dsdC+ product repressed its own synthesis. The repression was reversed by D-serine. dsdC expression was not depressed in a cya background. The basal level of D-serine deaminase was the same in wild-type and dsdCp fusion strains. The dsdC gene product was identified in maxicell strains harboring dsd plasmids as a 34,000-dalton protein. dsdC gene transcription proceeded clockwise; thus, its promoter is adjacent to that of dsdA.

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References

Curtiss 3rd R, Charamella L, Berg C, Harris P . Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli. J Bacteriol. 1965; 90(5):1238-50. PMC: 315808. DOI: 10.1128/jb.90.5.1238-1250.1965. View

Mandel M, Higa A . Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970; 53(1):159-62. DOI: 10.1016/0022-2836(70)90051-3. View

MCFALL E . Role of adenosine 3',5'-cyclic monophosphate and its specific binding protein in the regulation of D-serine deaminase synthesis. J Bacteriol. 1973; 113(2):781-5. PMC: 285293. DOI: 10.1128/jb.113.2.781-785.1973. View

Cosloy S . D-serine transport system in Escherichia coli K-12. J Bacteriol. 1973; 114(2):679-84. PMC: 251826. DOI: 10.1128/jb.114.2.679-684.1973. View

Cosloy S, MCFALL E . Metabolism of D-serine in Escherichia coli K-12: mechanism of growth inhibition. J Bacteriol. 1973; 114(2):685-94. PMC: 251827. DOI: 10.1128/jb.114.2.685-694.1973. View

Reznikoff W, Michels C, Cooper T, Silverstone A, MAGASANIK B . Inhibition of lacZ gene translation initiation in trp-lac fusion strains. J Bacteriol. 1974; 117(3):1231-9. PMC: 246606. DOI: 10.1128/jb.117.3.1231-1239.1974. View

Bloom F, MCFALL E, Young M, Carothers A . Positive control in the D-serine deaminase system of Escherichia coli K-12. J Bacteriol. 1975; 121(3):1092-101. PMC: 246040. DOI: 10.1128/jb.121.3.1092-1101.1975. View

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

Casadaban M . Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol. 1976; 104(3):557-66. DOI: 10.1016/0022-2836(76)90120-0. View

10.

Palchaudhuri S, MCFALL E, Carothers A . Specialized transduction of D-serine deaminase genes: formation of lysogens that yield high lambda-d dsd/lambda ratios and formation of a dimeric lambda-d dsd. J Bacteriol. 1976; 127(2):998-1014. PMC: 233010. DOI: 10.1128/jb.127.2.998-1014.1976. View

11.

Heincz M, MCFALL E . Role of small molecules in regulation of D-serine deaminase synthesis. J Bacteriol. 1978; 136(1):104-10. PMC: 218637. DOI: 10.1128/jb.136.1.104-110.1978. View

12.

Heincz M, MCFALL E . Role of the dsdC activator in regulation of D-serine deaminase synthesis. J Bacteriol. 1978; 136(1):96-103. PMC: 218636. DOI: 10.1128/jb.136.1.96-103.1978. View

13.

Eckhardt T . A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978; 1(4):584-8. DOI: 10.1016/0147-619x(78)90016-1. View

14.

Casadaban M, Cohen S . Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979; 76(9):4530-3. PMC: 411611. DOI: 10.1073/pnas.76.9.4530. View

15.

Carothers A, MCFALL E, Palchaudhuri S . Physical mapping of the Escherichia coli D-serine deaminase region: contiguity of the dsd structural and regulatory genes. J Bacteriol. 1980; 142(1):174-84. PMC: 293924. DOI: 10.1128/jb.142.1.174-184.1980. View

16.

Ogden S, Haggerty D, Stoner C, Kolodrubetz D, Schleif R . The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation. Proc Natl Acad Sci U S A. 1980; 77(6):3346-50. PMC: 349612. DOI: 10.1073/pnas.77.6.3346. View

17.

Lee N, Gielow W, Wallace R . Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(2):752-6. PMC: 319880. DOI: 10.1073/pnas.78.2.752. View

18.

Sancar A, Wharton R, Seltzer S, Kacinski B, Clarke N, RUPP W . Identification of the uvrA gene product. J Mol Biol. 1981; 148(1):45-62. DOI: 10.1016/0022-2836(81)90234-5. View

19.

Gray J, Patin D, Calhoun D . Identification of the protein products of the rrnC, ilv, rho region of the Escherichia coli K-12 chromosome. Mol Gen Genet. 1981; 183(3):428-36. DOI: 10.1007/BF00268761. View

20.

Kelley R, Yanofsky C . Trp aporepressor production is controlled by autogenous regulation and inefficient translation. Proc Natl Acad Sci U S A. 1982; 79(10):3120-4. PMC: 346365. DOI: 10.1073/pnas.79.10.3120. View