Molecular Characterization and Diagnostic Value of Taenia Solium Low-molecular-weight Antigen Genes

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2000 Dec 2

PMID 11101577

Citations 23

Authors

Y Sako

M Nakao

T Ikejima

X Z Piao

K Nakaya

A Ito

Affiliations

Soon will be listed here.

Abstract

Neurocysticercosis (NCC) caused by infection with the larvae of Taenia solium is an important cause of neurological disease worldwide. In order to establish an enzyme-linked immunosorbent assay (ELISA) for this infection using recombinant proteins, we carried out molecular cloning and identified four candidates as diagnostic antigens (designated Ag1, Ag1V1, Ag2, and Ag2V1). Except for Ag2V1, these clones could encode a 7-kDa polypeptide, and Ag2V1 could encode a 10-kDa polypeptide. All of the clones were very similar. Except for Ag2V1, recombinant proteins were successfully expressed using an Escherichia coli expression system. Immunoblot analysis of NCC patient sera detected recombinant proteins, but because reactivity to recombinant Ag1 was too weak, Ag1 was not suitable as an immunodiagnostic antigen. So, Ag1V1 and Ag2 were chosen as ELISA antigens, and the Ag1V1/Ag2 chimeric protein was expressed. Of 49 serum samples from NCC patients confirmed to be seropositive by immunoblot analysis, 44 (89.7%) were positive by ELISA. No assays of serum samples from patients with other parasitic infections recognized the Ag1V1/Ag2 chimeric protein. The Ag1V1/Ag2 chimeric protein obtained in this study had a high value for differential immunodiagnosis.

Citing Articles

Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis.

Sadaow L, Boonroumkaew P, Rodpai R, Janwan P, Sanpool O, Thanchomnang T Food Waterborne Parasitol. 2023; 33:e00211.

PMID: 37868190 PMC: 10589370. DOI: 10.1016/j.fawpar.2023.e00211.

Function of lipid binding proteins of parasitic helminths: still a long road.

Lombardo J, Porfido J, Sisti M, Giorello A, Rodriguez S, Corsico B Parasitol Res. 2022; 121(4):1117-1129.

PMID: 35169885 DOI: 10.1007/s00436-022-07463-1.

Expansion of a highly sensitive and specific ELISA test for diagnosis of hydatidosis using recombinant EgB8/2 protein.

Bashiri S, Nemati Mansoor F, Valadkhani Z Iran J Basic Med Sci. 2019; 22(2):134-139.

PMID: 30834077 PMC: 6396996. DOI: 10.22038/ijbms.2018.29024.7021.

Identification and Control of Sources of Infection - the Attempts To Eradicate the Parasite.

Samorek-Pierog M, Karamon J, Cencek T J Vet Res. 2018; 62(1):27-34.

PMID: 29978124 PMC: 5957458. DOI: 10.1515/jvetres-2018-0004.

Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis.

Guimaraes-Peixoto R, Pinto P, Santos M, Zilch T, Apolinario P, Silva-Junior A PLoS Negl Trop Dis. 2018; 12(4):e0006371.

PMID: 29649259 PMC: 6078323. DOI: 10.1371/journal.pntd.0006371.

References

Gottstein B, Tsang V, Schantz P . Demonstration of species-specific and cross-reactive components of Taenia solium metacestode antigens. Am J Trop Med Hyg. 1986; 35(2):308-13. DOI: 10.4269/ajtmh.1986.35.308. View

Hopp T, Woods K . A computer program for predicting protein antigenic determinants. Mol Immunol. 1983; 20(4):483-9. DOI: 10.1016/0161-5890(83)90029-9. View

Parkhouse R, HARRISON L . Cyst fluid and surface associated glycoprotein antigens of Taenia sp. metacestodes. Parasite Immunol. 1987; 9(2):263-8. DOI: 10.1111/j.1365-3024.1987.tb00505.x. View

Baily G, Mason P, Trijssenar F, Lyons N . Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg. 1988; 82(2):295-9. DOI: 10.1016/0035-9203(88)90451-8. View

Tsang V, Brand J, Boyer A . An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis. 1989; 159(1):50-9. DOI: 10.1093/infdis/159.1.50. View

Shepherd J, Aitken A, McManus D . A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. Mol Biochem Parasitol. 1991; 44(1):81-90. DOI: 10.1016/0166-6851(91)90223-s. View

Wilson M, Bryan R, Fried J, Ware D, Schantz P, Pilcher J . Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis. 1991; 164(5):1007-9. DOI: 10.1093/infdis/164.5.1007. View

Brandt J, Geerts S, De Deken R, Kumar V, Ceulemans F, Brijs L . A monoclonal antibody-based ELISA for the detection of circulating excretory-secretory antigens in Taenia saginata cysticercosis. Int J Parasitol. 1992; 22(4):471-7. DOI: 10.1016/0020-7519(92)90148-e. View

Schantz P, Moore A, Munoz J, Hartman B, Schaefer J, ARON A . Neurocysticercosis in an Orthodox Jewish community in New York City. N Engl J Med. 1992; 327(10):692-5. DOI: 10.1056/NEJM199209033271004. View

10.

Ohnishi K, Murata M, Nakane M, Takemura N, Tsuchida T, Nakamura T . Cerebral cysticercosis. Intern Med. 1993; 32(7):569-73. DOI: 10.2169/internalmedicine.32.569. View

11.

Zarlenga D, Rhoads M . A Taenia crassiceps cDNA sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis. Mol Biochem Parasitol. 1994; 67(2):215-23. DOI: 10.1016/0166-6851(94)00132-4. View

12.

Sloan L, Schneider S, Rosenblatt J . Evaluation of enzyme-linked immunoassay for serological diagnosis of cysticercosis. J Clin Microbiol. 1995; 33(12):3124-8. PMC: 228657. DOI: 10.1128/jcm.33.12.3124-3128.1995. View

13.

Craig P, Rogan M, Allan J . Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Adv Parasitol. 1996; 38:169-250. DOI: 10.1016/s0065-308x(08)60035-4. View

14.

Fernandez V, Ferreira H, Fernandez C, Zaha A, Nieto A . Molecular characterisation of a novel 8-kDa subunit of Echinococcus granulosus antigen B. Mol Biochem Parasitol. 1996; 77(2):247-50. DOI: 10.1016/0166-6851(96)02602-3. View

15.

Nielsen H, Engelbrecht J, Brunak S, von Heijne G . Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 1997; 10(1):1-6. DOI: 10.1093/protein/10.1.1. View

16.

White Jr A . Neurocysticercosis: a major cause of neurological disease worldwide. Clin Infect Dis. 1997; 24(2):101-13; quiz 114-5. DOI: 10.1093/clinids/24.2.101. View

17.

Ito A, Plancarte A, Ma L, Kong Y, Flisser A, Cho S . Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. Am J Trop Med Hyg. 1998; 59(2):291-4. DOI: 10.4269/ajtmh.1998.59.291. View

18.

Ohsaki Y, Matsumoto A, Miyamoto K, Kondoh N, Araki K, Ito A . Neurocysticercosis without detectable specific antibody. Intern Med. 1999; 38(1):67-70. DOI: 10.2169/internalmedicine.38.67. View

19.

Ito A, Ma L, Schantz P, Gottstein B, Liu Y, Chai J . Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). Am J Trop Med Hyg. 1999; 60(2):188-92. DOI: 10.4269/ajtmh.1999.60.188. View

20.

Hubert K, Andriantsimahavandy A, Michault A, Frosch M, Muhlschlegel F . Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci. Clin Diagn Lab Immunol. 1999; 6(4):479-82. PMC: 95711. DOI: 10.1128/CDLI.6.4.479-482.1999. View