Taenia Solium Metacestode Preparation in Rural Areas of Sub-Saharan Africa: a Source for Diagnosis and Research on Cysticercosis

Overview

Journal Afr Health Sci

Date 2015 Apr 3

PMID 25834531

Citations 2

Authors

V Schmidt

C S Sikasunge

E Odongo-Aginya

C Simukoko

G Mwanjali

S Alarakol

E Ovuga

W Matuja

C Kihamia

T Loscher

A S Winkler

G Bretzel

Affiliations

Soon will be listed here.

Abstract

Background: Taenia solium metacestodes/cysts obtained from pig carcasses constitute a primary source for diagnostic tools used for the detection of human cysticercosis. Data on T. solium cyst preparation in Africa is still scarce but required to establish independent reference laboratories.

Objectives: The aim of the present study is a) to present the likely yield of T. solium cyst material by the use of two different preparation methods in the field and b) to investigate its suitability for immunodiagnosis of human cysticercosis.

Methods: In Zambia, Uganda and Tanzania 670 pigs were screened for T. solium infection. Cysts were prepared by 'shaking method' and 'washing method'. Generated crude antigens were applied in a standard western blot assay.

Results: 46 out of 670 pigs (6.9%) were found positive for T. solium (Zambia: 12/367, 3.3%; Uganda: 11/217, 5.1%; Tanzania 23/86, 26.7%). Mean values of 77.7 ml whole cysts, 61.8 ml scolices/membranes and 10.9 ml cyst fluid were obtained per pig. Suitability of collected material for the use as crude antigen and molecular diagnostic techniques was demonstrated.

Conclusion: This study clearly shows that T. solium cyst preparation in African settings by simple field methods constitutes an effective way to obtain high quality material as source for diagnostic tools and research purposes.

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References

Dorny P, Phiri I, Vercruysse J, Gabriel S, Willingham 3rd A, Brandt J . A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. Int J Parasitol. 2004; 34(5):569-76. DOI: 10.1016/j.ijpara.2003.11.014. View

Deckers N, Dorny P . Immunodiagnosis of Taenia solium taeniosis/cysticercosis. Trends Parasitol. 2010; 26(3):137-44. DOI: 10.1016/j.pt.2009.12.008. View

Hancock K, Khan A, Williams F, Yushak M, Pattabhi S, Noh J . Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol. 2003; 41(6):2577-86. PMC: 156563. DOI: 10.1128/JCM.41.6.2577-2586.2003. View

Yang H, Chung J, Yun D, Kong Y, Ito A, Ma L . Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes. Parasite Immunol. 1998; 20(10):483-8. DOI: 10.1046/j.1365-3024.1998.00179.x. View

Bao M, Bogh H, Kassuku A, Nansen P . The prevalence of Taenia solium metacestodes in pigs in northern Tanzania. J Helminthol. 1995; 69(2):113-7. DOI: 10.1017/s0022149x00013997. View

Montano S, Villaran M, Ylquimiche L, Figueroa J, Rodriguez S, Bautista C . Neurocysticercosis: association between seizures, serology, and brain CT in rural Peru. Neurology. 2005; 65(2):229-33. DOI: 10.1212/01.wnl.0000168828.83461.09. View

Gottstein B, Tsang V, Schantz P . Demonstration of species-specific and cross-reactive components of Taenia solium metacestode antigens. Am J Trop Med Hyg. 1986; 35(2):308-13. DOI: 10.4269/ajtmh.1986.35.308. View

Sato M, Yamasaki H, Sako Y, Nakao M, Nakaya K, Plancarte A . Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen. Vet Parasitol. 2003; 111(4):309-22. DOI: 10.1016/s0304-4017(02)00383-7. View

Schagger H, von Jagow G . Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987; 166(2):368-79. DOI: 10.1016/0003-2697(87)90587-2. View

10.

Winkler A, Willingham 3rd A, Sikasunge C, Schmutzhard E . Epilepsy and neurocysticercosis in sub-Saharan Africa. Wien Klin Wochenschr. 2009; 121 Suppl 3:3-12. DOI: 10.1007/s00508-009-1242-3. View

11.

Mwape K, Phiri I, Praet N, Muma J, Zulu G, Van Den Bossche P . Taenia solium Infections in a rural area of Eastern Zambia-a community based study. PLoS Negl Trop Dis. 2012; 6(3):e1594. PMC: 3313923. DOI: 10.1371/journal.pntd.0001594. View

12.

Mwanjali G, Kihamia C, Kakoko D, Lekule F, Ngowi H, Johansen M . Prevalence and risk factors associated with human Taenia solium infections in Mbozi District, Mbeya Region, Tanzania. PLoS Negl Trop Dis. 2013; 7(3):e2102. PMC: 3597471. DOI: 10.1371/journal.pntd.0002102. View

13.

Winkler A . Neurocysticercosis in sub-Saharan Africa: a review of prevalence, clinical characteristics, diagnosis, and management. Pathog Glob Health. 2012; 106(5):261-74. PMC: 4005109. DOI: 10.1179/2047773212Y.0000000047. View

14.

Kanobana K, Praet N, Kabwe C, Dorny P, Lukanu P, Madinga J . High prevalence of Taenia solium cysticerosis in a village community of Bas-Congo, Democratic Republic of Congo. Int J Parasitol. 2011; 41(10):1015-8. DOI: 10.1016/j.ijpara.2011.06.004. View

15.

Nakao M, Okamoto M, Sako Y, Yamasaki H, Nakaya K, Ito A . A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide. Parasitology. 2002; 124(Pt 6):657-62. DOI: 10.1017/s0031182002001725. View

16.

Waiswa C, Fevre E, Nsadha Z, Sikasunge C, Willingham A . Porcine cysticercosis in southeast Uganda: seroprevalence in kamuli and kaliro districts. J Parasitol Res. 2010; 2009. PMC: 2915645. DOI: 10.1155/2009/375493. View

17.

Sikasunge C, Phiri I, Phiri A, Siziya S, Dorny P, Willingham 3rd A . Prevalence of Taenia solium porcine cysticercosis in the Eastern, Southern and Western provinces of Zambia. Vet J. 2007; 176(2):240-4. DOI: 10.1016/j.tvjl.2007.02.030. View

18.

Phiri I, Ngowi H, Afonso S, Matenga E, Boa M, Mukaratirwa S . The emergence of Taenia solium cysticercosis in Eastern and Southern Africa as a serious agricultural problem and public health risk. Acta Trop. 2003; 87(1):13-23. DOI: 10.1016/s0001-706x(03)00051-2. View

19.

Assana E, Kanobana K, Tume C, Zoli P, Nguekam , Geerts S . Isolation of a 14 kDa antigen from Taenia solium cyst fluid by HPLC and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis. Res Vet Sci. 2006; 82(3):370-6. DOI: 10.1016/j.rvsc.2006.09.006. View

20.

Ferrer E, Sanchez J, Milano A, Alvarez S, La Rosa R, Lares M . Diagnostic epitope variability within Taenia solium 8 kDa antigen family: implications for cysticercosis immunodetection. Exp Parasitol. 2011; 130(1):78-85. DOI: 10.1016/j.exppara.2011.10.010. View