Walt F Lima
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Explore the profile of Walt F Lima including associated specialties, affiliations and a list of published articles.
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Recent Articles
21.
Vickers T, Lima W, Nichols J, Crooke S
Nucleic Acids Res
. 2007 Oct;
35(19):6598-610.
PMID: 17905815
Administration of small interfering RNAs (siRNAs) leads to degradation of specific mRNAs utilizing the cellular RNA interference (RNAi) machinery. It has been demonstrated that co-administration of siRNAs may lead to...
22.
Lima W, Rose J, Nichols J, Wu H, Migawa M, Wyrzykiewicz T, et al.
Mol Pharmacol
. 2006 Oct;
71(1):83-91.
PMID: 17028158
In a previous study, we demonstrated that the sugar conformation and helical geometry of the heteroduplex substrate at the catalytic site of human RNase H1 directs the selective recognition of...
23.
Lima W, Rose J, Nichols J, Wu H, Migawa M, Wyrzykiewicz T, et al.
Mol Pharmacol
. 2006 Oct;
71(1):73-82.
PMID: 17028157
In a companion study published in this issue (p. 83), we showed that chimeric substrates containing 2'-methoxyethyl (MOE) nucleotides inhibited human RNase H1 activity. In this study, we prepared chimeric...
24.
Wang Z, Siwkowski A, Lima W, Olsen P, Ravikumar V
Bioorg Med Chem
. 2006 Mar;
14(14):5049-60.
PMID: 16563772
Multiple 2'-O-methoxyethyl modified phosphorothioate oligonucleotides of 18-20-mer in length were synthesized at various scales using 4,5-dicyanoimidazole (DCI) as coupling activator. Extensive synthetic, analytical (using ion-pair LC-MS), and in vivo pharmacological,...
25.
Lima W, Nichols J, Wu H, Prakash T, Migawa M, Wyrzykiewicz T, et al.
J Biol Chem
. 2004 Jun;
279(35):36317-26.
PMID: 15205459
Human RNase H1 cleaves RNA exclusively in an RNA/DNA duplex; neither double-strand DNA nor double-strand RNA is a viable substrate. Previous studies suggest that the helical geometry and sugar conformation...
26.
Ravikumar V, Lima W, Van Sooy K, Turney B
Nucleosides Nucleotides Nucleic Acids
. 2004 Mar;
23(1-2):149-60.
PMID: 15043143
Multiple phosphorothioate oligonucleotides containing a 3'-terminal negative charge were synthesized and characterized. Influence of the added negative charge on activation of duplexes by RNase H was investigated. No additional help...
27.
Wu H, Lima W, Zhang H, Fan A, Sun H, Crooke S
J Biol Chem
. 2004 Feb;
279(17):17181-9.
PMID: 14960586
Although ribonuclease H activity has long been implicated as a molecular mechanism by which DNA-like oligonucleotides induce degradation of target RNAs, definitive proof that one or more RNase H is...
28.
Ravikumar V, Capaldi D, Lima W, Lesnik E, Turney B, Cole D
Bioorg Med Chem
. 2003 Oct;
11(21):4673-9.
PMID: 14527564
A phosphorothioate-linked oligonucleotide bearing a 3'-terminal phosphorothioate monoester has been synthesized and characterized. This oligonucleotide has been identified as a process-related impurity formed during synthesis of ISIS 2302. Biological properties...
29.
Lima W, Wu H, Nichols J, Prakash T, Ravikumar V, Crooke S
J Biol Chem
. 2003 Sep;
278(50):49860-7.
PMID: 14506260
In a previous study, we showed that the RNA-binding domain of human RNase H1 is responsible for the positional preference for cleavage exhibited by the enzyme (Wu, H., Lima, W....
30.
Lima W, Wu H, Nichols J, Manalili S, Drader J, Hofstadler S, et al.
J Biol Chem
. 2002 Dec;
278(17):14906-12.
PMID: 12473655
Human RNase H1 is active only under reduced conditions. Oxidation as well as N-ethylmaleimide (NEM) treatment of human RNase H1 ablates the cleavage activity. The oxidized and NEM alkylated forms...