Thijs R H M Kouwen
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Explore the profile of Thijs R H M Kouwen including associated specialties, affiliations and a list of published articles.
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18
Citations
295
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Recent Articles
1.
McDonnell B, Mahony J, Hanemaaijer L, Kouwen T, Van Sinderen D
Appl Environ Microbiol
. 2017 Nov;
84(4).
PMID: 29180373
Predation of starter lactic acid bacteria such as by bacteriophages is a persistent and costly problem in the dairy industry. CRISPR-mediated acteriophage nsensitive utants (BIMs), while straightforward to generate and...
2.
Oliveira J, Mahony J, Hanemaaijer L, Kouwen T, Van Sinderen D
J Dairy Sci
. 2017 Nov;
101(1):96-105.
PMID: 29103710
In the current study, we characterized 137 Lactococcus lactis bacteriophages that had been isolated between 1997 and 2012 from whey samples obtained from industrial facilities located in 16 countries. Multiplex...
3.
Oliveira J, Mahony J, Hanemaaijer L, Kouwen T, Neve H, MacSharry J, et al.
Front Microbiol
. 2017 Aug;
8:1343.
PMID: 28769907
Most analyzed strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be...
4.
van der Ploeg R, Monteferrante C, Piersma S, Barnett J, Kouwen T, Robinson C, et al.
Appl Environ Microbiol
. 2012 Aug;
78(21):7733-44.
PMID: 22923407
The Gram-positive bacterium Bacillus subtilis contains two Tat translocases, which can facilitate transport of folded proteins across the plasma membrane. Previous research has shown that Tat-dependent protein secretion in B....
5.
van der Kooi-Pol M, Reilman E, Sibbald M, Veenstra-Kyuchukova Y, Kouwen T, Buist G, et al.
Appl Environ Microbiol
. 2012 Jul;
78(19):7124-7.
PMID: 22820325
Staphylococcus aureus is an important Gram-positive bacterial pathogen producing many secreted and cell surface-localized virulence factors. Here we report that the staphylococcal thiol-disulfide oxidoreductase DsbA is essential for stable biogenesis...
6.
Goosens V, Mars R, Akeroyd M, Vente A, Dreisbach A, Denham E, et al.
Antioxid Redox Signal
. 2012 May;
18(10):1159-64.
PMID: 22540663
The oxidative folding of proteins involves disulfide bond formation, which is usually catalyzed by thiol-disulfide oxidoreductases (TDORs). In bacteria, this process takes place in the cytoplasmic membrane and other extracytoplasmic...
7.
Miller M, Donat S, Rakette S, Stehle T, Kouwen T, Diks S, et al.
PLoS One
. 2010 Feb;
5(2):e9057.
PMID: 20140229
In eukaryotic cell types, virtually all cellular processes are under control of proline-directed kinases and especially MAP kinases. Serine/threonine kinases in general were originally considered as a eukaryote-specific enzyme family....
8.
Kouwen T, Nielsen A, Denham E, Dubois J, Dorenbos R, Rasmussen M, et al.
Appl Environ Microbiol
. 2009 Dec;
76(3):659-69.
PMID: 19948853
Bacillus subtilis is a well-established cell factory for efficient secretion of many biotechnologically relevant enzymes that are naturally produced by it or related organisms. However, the use of B. subtilis...
9.
Kouwen T, Trip E, Denham E, Sibbald M, Dubois J, van Dijl J
Antimicrob Agents Chemother
. 2009 Sep;
53(11):4702-11.
PMID: 19738010
Bacillus subtilis strain 168 produces the extremely stable and broad-spectrum lantibiotic sublancin 168. Known sublancin 168-susceptible organisms include important pathogens, such as Staphylococcus aureus. Nevertheless, since its discovery, the mode...
10.
Kouwen T, van Dijl J
Appl Microbiol Biotechnol
. 2009 Sep;
85(1):45-52.
PMID: 19727703
Bacillus subtilis is a well-established cellular factory for proteins and fine chemicals. In particular, the direct secretion of proteinaceous products into the growth medium greatly facilitates their downstream processing, which...