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T L Kirley

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Articles 54
Citations 573
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Recent Articles
1.
Kukulski F, Levesque S, Lavoie E, Lecka J, Bigonnesse F, Knowles A, et al.
Purinergic Signal . 2008 Apr; 1(2):193-204. PMID: 18404504
Nucleoside triphosphate diphosphohydrolases 1, 2, 3 and 8 (NTPDases 1, 2, 3 and 8) are the dominant ectonucleotidases and thereby expected to play important roles in nucleotide signaling. Distinct biochemical...
2.
Belcher S, Zsarnovszky A, Crawford P, Hemani H, Spurling L, Kirley T
Neuroscience . 2005 Dec; 137(4):1331-46. PMID: 16338080
Three anti-peptide antisera were raised against three distinct amino acid sequences of ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), characterized by Western blot analyses, and used to determine the distribution of NTPDase3...
3.
Kirley T, Yang F, Ivanenkov V
Arch Biochem Biophys . 2001 Oct; 395(1):94-102. PMID: 11673870
Glycine residues are recognized as important structural determinants in nucleotide-binding domains of many enzymes. The functional significance of seven glycine residues invariant in all 22 eNTPDase sequences was therefore examined....
4.
Yang F, Smith T, Kirley T
Biochim Biophys Acta . 2001 May; 1547(1):72-81. PMID: 11343793
Most ecto-nucleoside triphosphate diphosphohydrolases (eNTPDases) are inhibited by the histidine reagent diethyl pyrocarbonate (DEPC), while being resistant to inhibition by many other chemical modification agents. We used site-directed mutagenesis to...
5.
Yang F, Smith T, Kirley T
Biochemistry . 2001 Apr; 40(13):3943-50. PMID: 11300774
Ecto-nucleoside triphosphate diphosphohydrolase 3 (eNTPDase-3, also known as HB6 and CD39L3) is a membrane-associated ecto-apyrase. Only a few functionally significant residues have been elucidated for this enzyme, as well as...
6.
Kirley T
IUBMB Life . 2000 Nov; 50(1):43-50. PMID: 11087120
Human ecto-ATPase (ecto-nucleoside triphosphate diphosphohydrolase 2 [eNTPDase2], also known as CD39L1) has been expressed and characterized in COS cells. It exhibits some unusual enzymology that is similar to a few...
7.
Chadwick B, Frischauf A, Kirley T
J Biol Chem . 2000 Aug; 275(44):34041-5. PMID: 10948193
Ecto-nucleoside-triphosphate diphosphohydrolase-6 (eNTPDase6(1), also known as CD39L2) cDNA was expressed in mammalian COS-1 cells and characterized using nucleotidase assays as well as size exclusion, anion exchange, and cation exchange chromatography....
8.
Kirley T, Gerber L, Smith T
IUBMB Life . 2000 May; 48(1):67-72. PMID: 10791917
Chicken muscle ecto-ATPase has unusual enzyme kinetics and properties not found in many other E-type ATPases. To determine whether the unique properties of the chicken ecto-ATPase are inherent in the...
9.
Smith T, Lewis Carl S, Kirley T
Biochemistry . 1999 May; 38(18):5849-57. PMID: 10231536
A human brain E-type ATPase (HB6 ecto-apyrase) was subjected to site-directed mutagenesis to assess the functional significance of two highly conserved tryptophan residues (Trp 187 and Trp 459), the only...
10.
Smith T, Kirley T
Biochemistry . 1999 Feb; 38(5):1509-16. PMID: 9931016
The importance of N-linked glycosylation for the function and oligomerization of an E-type ATPase was examined by using tunicamycin and peptide N-glycosidase F, two agents used to prevent and remove...