T L Kirley
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Explore the profile of T L Kirley including associated specialties, affiliations and a list of published articles.
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54
Citations
573
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Recent Articles
11.
Smith T, Kirley T
Biochemistry
. 1999 Jan;
38(1):321-8.
PMID: 9890913
On the basis of sequence homologies observed between members of the E-type ATPases and the phosphate binding motifs of the actin/heat shock protein 70/sugar kinase superfamily, a human ecto-apyrase was...
12.
Smith T, Carl S, Kirley T
Biochem Mol Biol Int
. 1998 Sep;
45(5):1057-66.
PMID: 9739470
We have generated a polyclonal antibody (CKG2) against native chicken gizzard ecto-ATPase for immunolocalization and immunoprecipitation. Active ecto-ATPase is immunoprecipitated from solubilized chicken and rat membranes and shown to be...
13.
Smith T, Kirley T
Biochim Biophys Acta
. 1998 Jul;
1386(1):65-78.
PMID: 9675246
An extracellular ATPase (E-type ATPase) clone was isolated from a human brain cDNA library and sequenced. The transcript shows similarity to the previously published chicken smooth muscle and rat brain...
14.
Lewis Carl S, Smith T, Kirley T
Biochem Mol Biol Int
. 1998 Apr;
44(3):463-70.
PMID: 9556206
We have investigated the effect of cross-linking on the enzymatic activity and oligomer formation of the chicken stomach ecto-apyrase. Cross-linking with the hydrophobic, lysine-specific dithiobis(succinimidylpropionate) (DSP) caused equal inhibition of...
15.
Kirley T
J Biol Chem
. 1997 Sep;
272(38):23645-52.
PMID: 9295305
We have examined the in vivo localization of extracellular ecto-ATPase and ecto-apyrase (ATPDase) in adult chicken gizzard and stomach by immunofluorescence and laser scanning confocal microscopy. In chicken gizzard, the...
16.
Dombrowski K, Brewer K, Maleckar J, Kirley T, Thomas J, Kapp J
Arch Biochem Biophys
. 1997 Apr;
340(1):10-8.
PMID: 9126271
EctoATPases are extracellular membrane-bound enzymes that catalyze the hydrolysis of the gamma phosphate from ATP. EctoATPase is expressed by activated and immortalized Epstein-Barr virus-transformed human peripheral blood B lymphocytes and...
17.
Smith Jr T, Kirley T, Hennessey T
Arch Biochem Biophys
. 1997 Jan;
337(2):351-9.
PMID: 9016833
For the first time, a soluble, dedicated E-type ecto-ATPase has been identified and purified. This fully soluble ecto-ATPase is released into the growth media of the single-celled eukaryote, Tetrahymena, at...
18.
Kirley T
J Biol Chem
. 1997 Jan;
272(2):1076-81.
PMID: 8995405
The ecto-ATPase from chicken gizzard (smooth muscle) was solubilized, and the 66-kDa cell membrane ecto-ATPase protein was purified. The protein was then subjected to both enzymatic and chemical cleavage, and...
19.
Stout J, Kirley T
Biochemistry
. 1996 Jun;
35(25):8289-98.
PMID: 8679585
The extracellular ATPase (ecto-ATPase) is a divalent cation-dependent nucleoside triphosphatase with an unusually high specific activity. Monoclonal antibodies, described previously [Stout, J. G., Strobel, R. S., & Kirley, T. L....
20.
Stout J, Kirley T
Biochem Mol Biol Int
. 1995 Aug;
36(5):927-34.
PMID: 7581008
Several "specific" inhibitors of P2 purinergic receptors (purinoceptors) were evaluated for their ability to inhibit purified chicken gizzard cell membrane ecto-ATPase. The P2 purinoceptor antagonists tested included suramin, triazine based...