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Lorena S Beese

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Articles 51
Citations 2079
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Recent Articles
1.
Allert M, Kumar S, Wang Y, Beese L, Hellinga H
J Mol Biol . 2024 Sep; 436(22):168780. PMID: 39241982
ABC transporters are ancient and ubiquitous nutrient transport systems in bacteria and play a central role in defining lifestyles. Periplasmic solute-binding proteins (SBPs) are components that deliver ligands to their...
2.
Schey G, Hildebrandt E, Wang Y, Diwan S, Passetti H, Potts G, et al.
Int J Mol Sci . 2024 May; 25(10). PMID: 38791363
Protein farnesylation is a post-translational modification where a 15-carbon farnesyl isoprenoid is appended to the C-terminal end of a protein by farnesyltransferase (FTase). This process often causes proteins to associate...
3.
Allert M, Kumar S, Wang Y, Beese L, Hellinga H
Commun Chem . 2023 Aug; 6(1):168. PMID: 37598249
Fluorescent labeling of proteins is a powerful tool for probing structure-function relationships with many biosensing applications. Structure-based rules for systematically designing fluorescent biosensors require understanding ligand-mediated fluorescent response mechanisms which...
4.
Wang Y, Shi Y, Hellinga H, Beese L
Nucleic Acids Res . 2023 May; 51(11):5883-5894. PMID: 37166959
DNA polymerases are essential for nucleic acid synthesis, cloning, sequencing and molecular diagnostics technologies. Conditional intein splicing is a powerful tool for controlling enzyme reactions. We have engineered a thermal...
5.
Wang Y, Xu F, Nichols C, Shi Y, Hellinga H, Alspaugh J, et al.
J Med Chem . 2022 Oct; 65(20):13753-13770. PMID: 36218371
Infections by fungal pathogens are difficult to treat due to a paucity of antifungals and emerging resistances. Next-generation antifungals therefore are needed urgently. We have developed compounds that prevent farnesylation...
6.
Kato N, Kawasoe Y, Williams H, Coates E, Roy U, Shi Y, et al.
Cell Rep . 2017 Nov; 21(5):1375-1385. PMID: 29091773
DNA interstrand crosslinks (ICLs) that are repaired in non-dividing cells must be recognized independently of replication-associated DNA unwinding. Using cell-free extracts from Xenopus eggs that support neither replication nor transcription,...
7.
Shi Y, Hellinga H, Beese L
Proc Natl Acad Sci U S A . 2017 May; 114(23):6010-6015. PMID: 28533382
Human exonuclease 1 (hExo1) is a member of the RAD2/XPG structure-specific 5'-nuclease superfamily. Its dominant, processive 5'-3' exonuclease and secondary 5'-flap endonuclease activities participate in various DNA repair, recombination, and...
8.
Miller 3rd B, Beese L, Parish C, Wu E
Structure . 2015 Jul; 23(9):1609-1620. PMID: 26211612
DNA polymerases must quickly and accurately distinguish between similar nucleic acids to form Watson-Crick base pairs and avoid DNA replication errors. Deoxynucleoside triphosphate (dNTP) binding to the DNA polymerase active...
9.
Wang Y, Dozier J, Beese L, Distefano M
ACS Chem Biol . 2014 May; 9(8):1726-35. PMID: 24841702
Protein farnesytransferase (PFTase) catalyzes the farnesylation of proteins with a carboxy-terminal tetrapeptide sequence denoted as a Ca1a2X box. To explore the specificity of this enzyme, an important therapeutic target, solid-phase...
10.
Mabanglo M, Hast M, Lubock N, Hellinga H, Beese L
Protein Sci . 2013 Dec; 23(3):289-301. PMID: 24347326
Species of the fungal genus Aspergillus are significant human and agricultural pathogens that are often refractory to existing antifungal treatments. Protein farnesyltransferase (FTase), a critical enzyme in eukaryotes, is an...