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Jason T Boock

Explore the profile of Jason T Boock including associated specialties, affiliations and a list of published articles. Areas
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Articles 16
Citations 156
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Recent Articles
11.
Boock J, Waraho-Zhmayev D, Mizrachi D, DeLisa M
Methods Mol Biol . 2014 Dec; 1258:79-97. PMID: 25447860
Recombinant protein expression in Escherichia coli represents a cornerstone of the biotechnology enterprise. While cytoplasmic expression in this host has received the most attention, achieving substantial yields of correctly folded...
12.
Haitjema C, Boock J, Natarajan A, Dominguez M, Gardner J, Keating D, et al.
ACS Synth Biol . 2013 Nov; 3(2):74-82. PMID: 24200127
A variety of strategies now exist for the extracellular expression of recombinant proteins using laboratory strains of Escherichia coli . However, secreted proteins often accumulate in the culture medium at...
13.
Feiler C, Fisher A, Boock J, Marrichi M, Wright L, Schmidpeter P, et al.
PLoS One . 2013 Sep; 8(9):e73123. PMID: 24023821
Directed evolution can be a powerful tool for revealing the mutational pathways that lead to more resistant bacterial strains. In this study, we focused on the bacterium Mycobacterium tuberculosis, which...
14.
Contreras-Martinez L, Boock J, Kostecki J, DeLisa M
Biotechnol J . 2011 Nov; 7(3):354-60. PMID: 22076828
The folding of many cellular proteins occurs co-translationally immediately outside the ribosome exit tunnel, where ribosomal proteins and other associated factors coordinate the synthesis and folding of newly translated polypeptides....
15.
Conrado R, Wu G, Boock J, Xu H, Chen S, Lebar T, et al.
Nucleic Acids Res . 2011 Oct; 40(4):1879-89. PMID: 22021385
Synthetic scaffolds that permit spatial and temporal organization of enzymes in living cells are a promising post-translational strategy for controlling the flow of information in both metabolic and signaling pathways....
16.
Liang J, Kim J, Boock J, Mansell T, Ostermeier M
Protein Sci . 2007 Apr; 16(5):929-37. PMID: 17400921
A heterotropic allosteric effect involves an effector molecule that is distinct from the substrate or ligand of the protein. How heterotropic allostery originates is an unanswered question. We have previously...