J R Silvius
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Explore the profile of J R Silvius including associated specialties, affiliations and a list of published articles.
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Articles
78
Citations
1332
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Recent Articles
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Sang S, Silvius J
J Pept Res
. 2005 Sep;
66(4):169-80.
PMID: 16138855
S-acylated peptides have many potential uses for elucidating the biophysical, structural and other properties of the numerous S-acylated proteins of mammalian cells. However, with the currently available reagents, preparation of...
3.
Silvius J
J Membr Biol
. 2002 Dec;
190(2):83-92.
PMID: 12474073
Many physiological and oncogenic activities of the "classical" Ras proteins (H-Ras, N-Ras and K-Ras4A and -4B) require their correct localization to the plasma membrane. Nascent Ras proteins, however, initially associate...
4.
Wang T, Leventis R, Silvius J
Biochemistry
. 2001 Oct;
40(43):13031-40.
PMID: 11669641
We have used a fluorescence assay and detergent fractionation to examine the partitioning of different fluorescent lipidated peptides, with sequences and lipid substituents matching those found in various classes of...
5.
Wang T, Silvius J
Biophys J
. 2001 Oct;
81(5):2762-73.
PMID: 11606289
A fluorescence-quenching method has been used to assess the potential formation of segregated liquid-ordered domains in lipid bilayers combining cholesterol with mixtures of amino and choline phospholipids like those found...
6.
Leventis R, Silvius J
Biophys J
. 2001 Sep;
81(4):2257-67.
PMID: 11566796
In view of the demonstrated cholesterol-binding capabilities of certain cyclodextrins, we have examined whether these agents can also catalyze efficient transfer of cholesterol between lipid vesicles. We here demonstrate that...
7.
Wang T, Silvius J
Biophys J
. 2000 Sep;
79(3):1478-89.
PMID: 10969009
Two fluorescence-based approaches have been applied to examine the differential partitioning of fluorescent phospho- and sphingolipid molecules into sphingolipid-enriched domains modeling membrane "lipid rafts." Fluorescence-quenching measurements reveal that N-(diphenylhexatrienyl)propionyl- (DPH3:0-)-labeled...
8.
Wang T, Leventis R, Silvius J
Biophys J
. 2000 Aug;
79(2):919-33.
PMID: 10920023
A fluorescence-quenching assay is described that can directly monitor the relative extents of partitioning of different but structurally homologous fluorescent molecules into liquid-ordered (l(o)) domains in lipid vesicles exhibiting liquid-ordered/liquid-disordered...
9.
Roy M, Leventis R, Silvius J
Biochemistry
. 2000 Jul;
39(28):8298-307.
PMID: 10889039
Mutational analysis and in vitro assays of membrane association have been combined to investigate the mechanism of plasma membrane targeting mediated by the farnesylated, polybasic carboxy-terminal sequence of K-ras4B in...
10.
Dunphy J, Schroeder H, Leventis R, Greentree W, Knudsen J, Silvius J, et al.
Biochim Biophys Acta
. 2000 Jun;
1485(2-3):185-98.
PMID: 10832099
Both enzymatic and autocatalytic mechanisms have been proposed to account for protein thioacylation (commonly known as palmitoylation). Acyl-CoA binding proteins (ACBP) strongly suppress non-enzymatic thioacylation of cysteinyl-containing peptides by long-chain...