J David Barrass
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Explore the profile of J David Barrass including associated specialties, affiliations and a list of published articles.
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18
Citations
548
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Recent Articles
1.
Mendoza-Ochoa G, Barrass J, Maudlin I, Beggs J
RNA Biol
. 2019 Nov;
16(12):1775-1784.
PMID: 31671032
Pre-messenger RNA splicing involves multi-step assembly of the large spliceosome complexes that catalyse the two consecutive trans-esterification reactions, resulting in intron removal. There is evidence that proof-reading mechanisms monitor the...
2.
Barrass J, Beggs J
J Vis Exp
. 2019 Sep;
(150).
PMID: 31498317
The nucleotide analogue, 4-thiouracil (4tU), is readily taken up by cells and incorporated into RNA as it is transcribed in vivo, allowing isolation of the RNA produced during a brief...
3.
Barrass J, Mendoza-Ochoa G, Maudlin I, Sani E, Beggs J
J Vis Exp
. 2019 Aug;
(149).
PMID: 31380835
The plant auxin binding receptor, TIR1, recognizes proteins containing a specific auxin-inducible degron (AID) motif in the presence of auxin, targeting them for degradation. This system is exploited in many...
4.
Mendoza-Ochoa G, Barrass J, Terlouw B, Maudlin I, de Lucas S, Sani E, et al.
Yeast
. 2018 Oct;
36(1):75-81.
PMID: 30375036
The auxin-inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell...
5.
Tudek A, Schmid M, Makaras M, Barrass J, Beggs J, Jensen T
Cell Rep
. 2018 Aug;
24(9):2457-2467.e7.
PMID: 30157437
Genomes are promiscuously transcribed, necessitating mechanisms that facilitate the sorting of RNA for function or destruction. The polyA (pA) tail is one such distinguishing feature, which in the Saccharomyces cerevisiae...
6.
Barrass J, Reid J, Huang Y, Hector R, Sanguinetti G, Beggs J, et al.
Genome Biol
. 2015 Dec;
16:282.
PMID: 26679539
Background: RNA levels detected at steady state are the consequence of multiple dynamic processes within the cell. In addition to synthesis and decay, transcripts undergo processing. Metabolic tagging with a...
7.
Gautam A, Grainger R, Vilardell J, Barrass J, Beggs J
Nucleic Acids Res
. 2015 Mar;
43(6):3309-17.
PMID: 25740649
Pre-mRNA splicing involves two transesterification steps catalyzed by the spliceosome. How RNA substrates are positioned in each step and the molecular rearrangements involved, remain obscure. Here, we show that mutations...
8.
Cordin O, Hahn D, Alexander R, Gautam A, Saveanu C, Barrass J, et al.
Nucleic Acids Res
. 2014 Nov;
42(22):13897-910.
PMID: 25428373
RNA helicases are essential for virtually all cellular processes, however, their regulation is poorly understood. The activities of eight RNA helicases are required for pre-mRNA splicing. Amongst these, Brr2p is...
9.
Chathoth K, Barrass J, Webb S, Beggs J
Mol Cell
. 2014 Feb;
53(5):779-90.
PMID: 24560925
There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown. Prp5 protein (Prp5p) is an RNA-stimulated adenosine...
10.
Swiatkowska A, Wlotzka W, Tuck A, Barrass J, Beggs J, Tollervey D
RNA
. 2012 Oct;
18(12):2187-200.
PMID: 23093724
Pre-ribosomal particles undergo numerous structural changes during maturation, but their high complexity and short lifetimes make these changes very difficult to follow in vivo. In consequence, pre-ribosome structure and composition...