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Hiroya Tomita

Explore the profile of Hiroya Tomita including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 27
Citations 201
Followers 0
Related Specialties
Biochemistry
Microbiology
Molecular Biology
Top 10 Co-Authors
Haruyuki Atomi
Tadayuki Imanaka
Yuusuke Yokooji
Kenichiro Matsumoto
Takuya Ishibashi
Kunio Miki
Yasuo Ohnishi
Yohei Katsuyama
Akiko Kita
Asako Kishimoto
Published In
J Bacteriol
J Biol Chem
Proteins
Chembiochem
Biomacromolecules
Affiliations
Soon will be listed here.
Recent Articles
1.
KEIO knockout collection reveals metabolomic crosstalk in Chlorella spp.-Escherichia coli co-cultures
Tong C, Tomita H, Miyazaki K, Derek C, Honda K
J Phycol . 2025 Mar; PMID: 40074247
The interdependence between microalgae and bacteria has sparked scientific interest over years, primarily driven by the practical applications of microalgal-bacteria consortia in wastewater treatment and algal biofuel production. Although adequate...
2.
Development of an enzymatic cascade for semi de novo ATP production using thermophilic enzymes
Suzuki T, Gladwin S, Miyazaki K, Tomita H, Honda K
J Biosci Bioeng . 2025 Mar; PMID: 40064564
Industrial production of ATP has mostly relied on extraction from living cells. Although microbial and enzymatic ATP production have also been developed, the former suffers from complexity in product separation,...
3.
Biosynthetic Characterization of Bacillibactin in Thermophilic Bacillaceae and its Potential for in Vitro Mutational Synthesis
Izumi M, Tomita H, Miyazaki K, Otsuka R, Honda K
Chembiochem . 2025 Jan; 26(5):e202400836. PMID: 39870587
Bacillibactin (BB) is a microbial siderophore produced by Bacillus species. BB is biosynthesized from 2,3-dihydroxybenzoic acid (2,3-DHB), Gly, and L-Thr by nonribosomal peptide synthetase (NRPS) enzymes DhbE, DhbB, and DhbF....
4.
Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase
Kawakami T, Tomita H, Hien P, Matsumoto K
Biopolymers . 2024 Aug; 115(6):e23618. PMID: 39162134
This study synthesized poly(3-hydroxypropionate) [P(3HP)]-containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)-b-P[2-hydroxybutyrate (2HB)] and P(3HP)-b-P(D-lactate) (PDLA), using Escherichia coli. The cells expressing an evolved sequence-regulating PHA synthase, PhaCNDFH, and propionyl-CoA transferase were...
5.
Engineering of the Long-Main-Chain Monomer-Incorporating Polyhydroxyalkanoate Synthase PhaC for the Biosynthesis of Poly[()-3-hydroxybutyrate--6-hydroxyhexanoate]
Hozumi Y, Hachisuka S, Tomita H, Kikukawa H, Matsumoto K
Biomacromolecules . 2024 Apr; 25(5):2973-2979. PMID: 38588330
Polyhydroxyalkanoate (PHA) synthases (PhaCs) are useful and versatile tools for the production of aliphatic polyesters. Here, the chimeric PHA synthase PhaC was engineered to increase its capacity to incorporate unusual...
6.
Real-time NMR analysis of polyhydroxyalkanoate synthase reaction that synthesizes block copolymer comprising glycolate and 3-hydroxybutyrate
Yanagawa K, Kajikawa A, Sakakibara S, Kumeta H, Tomita H, Matsumoto K
Biophys Chem . 2023 Mar; 296:107001. PMID: 36913888
The sequence-regulating polyhydroxyalkanoate (PHA) synthase PhaC spontaneously synthesizes the homo-random block copolymer, poly[3-hydroxybutyrate (3HB)]-b-poly[glycolate (GL)-ran-3HB]. In this study, a real-time in vitro chasing system was established using a high-resolution 800 ...
7.
Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaC
Satoh K, Kawakami T, Isobe N, Pasquier L, Tomita H, Zinn M, et al.
Microb Cell Fact . 2022 May; 21(1):84. PMID: 35568875
Background: Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaC, a unique sequence-regulating PHA synthase, has enabled...
8.
Directed Evolution of Sequence-Regulating Polyhydroxyalkanoate Synthase to Synthesize a Medium-Chain-Length-Short-Chain-Length (MCL-SCL) Block Copolymer
Phan H, Hosoe Y, Guex M, Tomoi M, Tomita H, Zinn M, et al.
Biomacromolecules . 2022 Jan; 23(3):1221-1231. PMID: 34991313
Sequence-regulating polyhydroxyalkanoate synthase PhaC is a chimeric enzyme comprising PhaCs from and (). It spontaneously synthesizes a short-chain-length (SCL, ≤C) block copolymer poly(2-hydroxybutyrate)--poly(3-hydroxybutyrate) [P(2HB)--P(3HB)] from a mixture of monomer substrates....
9.
Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material
Kageyama Y, Tomita H, Isono T, Satoh T, Matsumoto K
Sci Rep . 2021 Nov; 11(1):22446. PMID: 34789822
The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaC with monomer sequence-regulating capacity. In the present study, the...
10.
Biosynthesis of poly(glycolate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate) in Escherichia coli expressing sequence-regulating polyhydroxyalkanoate synthase and medium-chain-length 3-hydroxyalkanoic acid coenzyme A ligase
Tomita H, Satoh K, Nomura C, Matsumoto K
Biosci Biotechnol Biochem . 2021 Nov; 86(2):217-223. PMID: 34788370
Chimeric polyhydroxyalkanoate synthase PhaCAR is characterized by the capacity to incorporate unusual glycolate (GL) units and spontaneously synthesize block copolymers. The GL and 3-hydroxybutyrate (3HB) copolymer synthesized by PhaCAR is...