Alexander Hillar
Overview
Explore the profile of Alexander Hillar including associated specialties, affiliations and a list of published articles.
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Articles
7
Citations
77
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Recent Articles
1.
Aumiller J, Mabashi-Asazuma H, Hillar A, Shi X, Jarvis D
Glycobiology
. 2011 Nov;
22(3):417-28.
PMID: 22042767
The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems. In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering...
2.
Hillar A, Jarvis D
Glycobiology
. 2010 Jun;
20(10):1323-30.
PMID: 20574041
It was previously reported that Tn-4h and DpN1 cells have the endogenous capacity to efficiently sialylate secreted alkaline phosphatase (SEAP) when infected with a baculovirus expression vector. In contrast, it...
3.
Hillar A, Otulakowski G, O Brodovich H
Protein Expr Purif
. 2007 Aug;
55(2):246-56.
PMID: 17681809
To generate hemoglobin-free full-length haptoglobin the cDNA encoding rat haptoglobin alphabeta subunits was cloned into shuttle vector pVT-Bac-His and used to produce a recombinant baculovirus Autographa californica Nuclear Polyhedrosis Virus...
4.
Hillar A, Culham D, Vernikovska Y, Wood J, Boggs J
Biochemistry
. 2005 Jul;
44(30):10170-80.
PMID: 16042394
Membrane transporter ProP from Escherichia coli senses extracellular osmolality and responds by mediating the uptake of osmoprotectants such as glycine betaine when osmolality is high. Earlier EPR and NMR studies...
5.
Wood J, Culham D, Hillar A, Vernikovska Y, Liu F, Boggs J, et al.
Biochemistry
. 2005 Apr;
44(15):5634-46.
PMID: 15823022
Transporter ProP of Escherichia coli, a member of the major facilitator superfamily (MFS), acts as an osmosensor and an osmoregulator in cells and after purification and reconstitution in proteoliposomes. H(+)-osmoprotectant...
6.
Hillar A, Tripet B, Zoetewey D, Wood J, Hodges R, Boggs J
Biochemistry
. 2003 Dec;
42(51):15170-8.
PMID: 14690427
Electron paramagnetic resonance spectroscopy was used to determine relative peptide orientation within homodimeric, alpha-helical coiled-coil structures. Introduction of cysteine (Cys) residues into peptides/proteins for spin labeling allows detection of their...
7.
Culham D, Hillar A, Henderson J, Ly A, Vernikovska Y, Racher K, et al.
Biochemistry
. 2003 Oct;
42(40):11815-23.
PMID: 14529293
Transporter ProP of Escherichia coli is an osmosensor and an osmoprotectant transporter. Previous results suggest that medium osmolality determines the proportions of ProP in active and inactive conformations. A cysteine-less...