Alpha 2.0
Login Register
» Articles » PMID: 984767

New Plasmid-mediated Nucleotidylation of Aminoglycoside Antibiotics in Staphlococcus Aureus

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 1976 Aug 1
PMID 984767
Citations 22
Authors
F Le Goffic
A Martel
M L Capmau
B Baca
P Goebel
H Chardon
C J Soussy
J Duval
D H Bouanchaud
Affiliations
Soon will be listed here.
Abstract

A wild-type strain of Staphylococcus aureus, which inactivates a wide variety of aminoglycosides (except the gentamicin components), has been found to harbor a plasmid (RAp01) that mediates the biosynthesis of a nucleotidyltransferase. This enzyme modifies the 4'-hydroxy function of these antibiotics. The plasmid has been studied, the enzyme responsible for this resistance pattern has been isolated by affinity chromatography, and its kinetics and physicochemistry have been characterized. The target of this enzyme has also been located by demonstrating the structure of one inactivated compound, 4'-(O)-adenylyltobramycin.

Citing Articles

"Catch and Release": A Variation of the Archetypal Nucleotidyl Transfer Reaction.

Selvaraj B, Kocaman S, Trifas M, Serpersu E, Cuneo M ACS Catal. 2024; 10(6):3548-3555.

PMID: 38250052 PMC: 10797667. DOI: 10.1021/acscatal.9b05201.

Self-Resistance during Muraymycin Biosynthesis: a Complementary Nucleotidyltransferase and Phosphotransferase with Identical Modification Sites and Distinct Temporal Order.

Cui Z, Wang X, Liu X, Lemke A, Koppermann S, Ducho C Antimicrob Agents Chemother. 2018; 62(7).

PMID: 29735559 PMC: 6021665. DOI: 10.1128/AAC.00193-18.

Crystal structure and kinetic mechanism of aminoglycoside phosphotransferase-2''-IVa.

Toth M, Frase H, Antunes N, Smith C, Vakulenko S Protein Sci. 2010; 19(8):1565-76.

PMID: 20556826 PMC: 2923509. DOI: 10.1002/pro.437.

Metabolite profiling uncovers plasmid-induced cobalt limitation under methylotrophic growth conditions.

Kiefer P, Buchhaupt M, Christen P, Kaup B, Schrader J, Vorholt J PLoS One. 2009; 4(11):e7831.

PMID: 19915676 PMC: 2773004. DOI: 10.1371/journal.pone.0007831.

Aminoglycoside resistance gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria.

Sabtcheva S, Galimand M, Gerbaud G, Courvalin P, Lambert T Antimicrob Agents Chemother. 2003; 47(5):1584-8.

PMID: 12709326 PMC: 153341. DOI: 10.1128/AAC.47.5.1584-1588.2003.

References
1.
Kobayashi F, Koshi T, Eda J, Yoshimura Y, Mitsuhashi S . Lividomycin resistance in staphylococci by enzymatic phosphorylation. Antimicrob Agents Chemother. 1973; 4(1):1-5. PMC: 444495. DOI: 10.1128/AAC.4.1.1. View
2.
Kondo S, Yamamoto H, Naganawa H, Umezawa H . Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor. J Antibiot (Tokyo). 1972; 25(8):483-4. DOI: 10.7164/antibiotics.25.483. View
3.
Le Goffic F, Moreau N . Purification by affinity chromatography of an enzyme involved in gentamicin inactivation. FEBS Lett. 1973; 29(3):289-291. DOI: 10.1016/0014-5793(73)80041-9. View
4.
Doi O, Miyamoto M, Tanaka N, Umezawa H . Inactivation and phosphorylation of kanamycin by drug-resistant Staphylococcus aureus. Appl Microbiol. 1968; 16(9):1282-4. PMC: 547641. DOI: 10.1128/am.16.9.1282-1284.1968. View
5.
Benveniste R, Davies J . Mechanisms of antibiotic resistance in bacteria. Annu Rev Biochem. 1973; 42:471-506. DOI: 10.1146/annurev.bi.42.070173.002351. View