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3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by Escherichia Coli Carrying an R Factor

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 1974 Dec 1
PMID 4615629
Citations 12
Authors
F Le Goffic
A Martel
J Witchitz
Affiliations
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Abstract

A new R factor-mediated gentamicin acetyltransferase obtained from a clinical isolate of gentamicin-, tobramycin-, and kanamycin-resistant Klebsiella was characterized. R factor R 176 was transferred to Escherichia coli K-12 LA 290 from a Klebsiella. Resistance transfer, minimal inhibitory concentrations of various aminoglycoside antibiotics, enzymatic inactivation, and the isoelectric point of the enzyme obtained were determined. We found that R factor R 176 could govern the enzymatic 3-N acetylation of gentamicin, tobramycin, and kanamycin, and isoelectric point determinations suggested that the enzymes governed by R 176 and R 135 are different. The genes responsible for resistance to gentamicin, tobramycin, and kanamycin govern the synthesis of an isoenzyme of AAC(3)I, which we propose to call AAC(3)II.

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References
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Chevereau M, Daniels P, Davies J, LeGoffic F . Aminoglycoside resistance in bacteria medicated by gentamicin acetyltransferase II, an enzyme modifying the 2'-amino group of aminoglycoside antibiotics. Biochemistry. 1974; 13(3):598-603. DOI: 10.1021/bi00700a030. View
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