Genetic Basis for Clarithromycin Resistance Among Isolates of Mycobacterium Chelonae and Mycobacterium Abscessus

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1996 Jul 1

PMID 8807061

Citations 124

Authors

R J Wallace Jr

A Meier

B A Brown

Y Zhang

P Sander

G O Onyi

E C Bottger

Affiliations

Soon will be listed here.

Abstract

Resistance to clarithromycin among isolates of Mycobacterium chelonae and M. abscessus was observed in 18 of 800 (2.3%) patients tested between 1990 and 1995. Patients whose isolates were resistant had either disseminated disease or chronic lung disease, and the resistant isolates were recovered after clarithromycin monotherapy. Sequencing of the gene coding for the 23S rRNA peptidyltransferase region revealed a point mutation involving adenine at position 2058 (38%) or adenine at position 2059 (62%) in 20 of 20 relapse isolates from the first 13 patients identified. By pulsed-field gel electrophoresis or random amplified polymorphic DNA PCR, initial and relapse isolates were shown to have identical DNA patterns. M. chelonae and M. abscessus isolates were found to have only a single chromosomal copy of the rRNA operon, thus making them susceptible to single-step mutations. Thus, clarithromycin resistance in these species of rapidly growing mycobacteria relates to a point mutation in the gene coding for 23S rRNA and occurs in limited clinical situations, but was identified in almost 5% of isolates tested in 1995.

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References

Steingrube V, Wallace Jr R, Steele L, Pang Y . Mercuric reductase activity and evidence of broad-spectrum mercury resistance among clinical isolates of rapidly growing mycobacteria. Antimicrob Agents Chemother. 1991; 35(5):819-23. PMC: 245113. DOI: 10.1128/AAC.35.5.819. View

Versalovic J, Shortridge D, Kibler K, Griffy M, Beyer J, Flamm R . Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrob Agents Chemother. 1996; 40(2):477-80. PMC: 163139. DOI: 10.1128/AAC.40.2.477. View

Young L, Wiviott L, Wu M, Kolonoski P, Bolan R, Inderlied C . Azithromycin for treatment of Mycobacterium avium-intracellulare complex infection in patients with AIDS. Lancet. 1991; 338(8775):1107-9. DOI: 10.1016/0140-6736(91)91965-w. View

Silcox V, Good R, Floyd M . Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol. 1981; 14(6):686-91. PMC: 274023. DOI: 10.1128/jcm.14.6.686-691.1981. View

Brown B, Wallace Jr R, Onyi G, De Rosas V, Wallace 3rd R . Activities of four macrolides, including clarithromycin, against Mycobacterium fortuitum, Mycobacterium chelonae, and M. chelonae-like organisms. Antimicrob Agents Chemother. 1992; 36(1):180-4. PMC: 189249. DOI: 10.1128/AAC.36.1.180. View

Brown B, Wallace Jr R, Onyi G . Activities of clarithromycin against eight slowly growing species of nontuberculous mycobacteria, determined by using a broth microdilution MIC system. Antimicrob Agents Chemother. 1992; 36(9):1987-90. PMC: 192220. DOI: 10.1128/AAC.36.9.1987. View

Wallace Jr R, Swenson J, Silcox V, Good R, Tschen J, Stone M . Spectrum of disease due to rapidly growing mycobacteria. Rev Infect Dis. 1983; 5(4):657-79. DOI: 10.1093/clinids/5.4.657. View

Wallace Jr R, Hull S, Bobey D, PRICE K, Swenson J, Steele L . Mutational resistance as the mechanism of acquired drug resistance to aminoglycosides and antibacterial agents in Mycobacterium fortuitum and Mycobacterium chelonei. Evidence is based on plasmid analysis, mutational frequencies, and.... Am Rev Respir Dis. 1985; 132(2):409-16. DOI: 10.1164/arrd.1985.132.2.409. View

Swenson J, Wallace Jr R, Silcox V, Thornsberry C . Antimicrobial susceptibility of five subgroups of Mycobacterium fortuitum and Mycobacterium chelonae. Antimicrob Agents Chemother. 1985; 28(6):807-11. PMC: 180333. DOI: 10.1128/AAC.28.6.807. View

10.

Bercovier H, Kafri O, Sela S . Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys Res Commun. 1986; 136(3):1136-41. DOI: 10.1016/0006-291x(86)90452-3. View

11.

Pernodet J, Boccard F, Alegre M, Guerineau M . Resistance to macrolides, lincosamides and streptogramin type B antibiotics due to a mutation in an rRNA operon of Streptomyces ambofaciens. EMBO J. 1988; 7(1):277-82. PMC: 454269. DOI: 10.1002/j.1460-2075.1988.tb02810.x. View

12.

Dautzenberg B, Saint Marc T, Meyohas M, Eliaszewitch M, Haniez F, Rogues A . Clarithromycin and other antimicrobial agents in the treatment of disseminated Mycobacterium avium infections in patients with acquired immunodeficiency syndrome. Arch Intern Med. 1993; 153(3):368-72. View

13.

Mazurek G, Hartman S, Zhang Y, Brown B, Hector J, Murphy D . Large DNA restriction fragment polymorphism in the Mycobacterium avium-M. intracellulare complex: a potential epidemiologic tool. J Clin Microbiol. 1993; 31(2):390-4. PMC: 262771. DOI: 10.1128/jcm.31.2.390-394.1993. View

14.

Swetter S, Kindel S, Smoller B . Cutaneous nodules of Mycobacterium chelonae in an immunosuppressed patient with preexisting pulmonary colonization. J Am Acad Dermatol. 1993; 28(2 Pt 2):352-5. DOI: 10.1016/0190-9622(93)70053-v. View

15.

Nadal D, Caduff R, Kraft R, Salfinger M, Bodmer T, Kirschner P . Invasive infection with Mycobacterium genavense in three children with the acquired immunodeficiency syndrome. Eur J Clin Microbiol Infect Dis. 1993; 12(1):37-43. DOI: 10.1007/BF01997055. View

16.

Wallace Jr R, Tanner D, Brennan P, Brown B . Clinical trial of clarithromycin for cutaneous (disseminated) infection due to Mycobacterium chelonae. Ann Intern Med. 1993; 119(6):482-6. DOI: 10.7326/0003-4819-119-6-199309150-00006. View

17.

Wallace Jr R, Zhang Y, Brown B, Fraser V, Mazurek G, Maloney S . DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus. J Clin Microbiol. 1993; 31(10):2697-701. PMC: 265980. DOI: 10.1128/jcm.31.10.2697-2701.1993. View

18.

Heifets L, Mor N, Vanderkolk J . Mycobacterium avium strains resistant to clarithromycin and azithromycin. Antimicrob Agents Chemother. 1993; 37(11):2364-70. PMC: 192393. DOI: 10.1128/AAC.37.11.2364. View

19.

Jacobs I, Potsic W . Glomus tympanicum in infancy. Arch Otolaryngol Head Neck Surg. 1994; 120(2):203-5. DOI: 10.1001/archotol.1994.01880260073013. View

20.

Domenech P, Menendez M, Garcia M . Restriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast-growing mycobacterial species. FEMS Microbiol Lett. 1994; 116(1):19-24. DOI: 10.1111/j.1574-6968.1994.tb06669.x. View