Novel Synthetic Peptide Agelaia-12 Has Improved Activity Against Complex

Overview

Journal Pathogens

Date 2024 Nov 27

PMID 39599547

Authors

Arthur Alves Coelho

Lilia Cristina de Souza Barbosa

Adeliane Castro da Costa

Andre Kipnis

Ana Paula Junqueira-Kipnis

Affiliations

Soon will be listed here.

Abstract

Fast-growing mycobacteria cause difficult-to-treat infections due to their high intrinsic resistance to antibiotics as well as disinfectant agents. complex (MAC) is the main cause of nontuberculous mycobacteria diseases. In this work, we evaluated the activity of the novel synthetic antimicrobial peptide, Agelaia-12, against and . Agelaia-12 showed a minimum inhibitory concentration (MIC) of 25 μM detected against and with no cytotoxicity. The scanning electronic microscopy analysis of mycobacterial treated with Agelaia-12 demonstrated the presence of filamentous structures and aggregation of the cells. Congo red binding assay of exhibited altered dye accumulation after treatment with Agelaia-12. Treatment of - or -infected murine macrophages with Agelaia-12 decreased the mycobacterial load by 92% for the tested strains. Additionally, IFN-y KO mice infected with or and treated with Agelaia-12 showed a 98% reduction in lung bacterial load. Thus, the synthetic peptide Agelaia-12 may be a promising biomolecule for the treatment of mycobacteriosis, and its structural properties may serve as a foundational model for the design and development of novel pharmaceutical agents aimed at combating this disease.

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