Restriction Fragment Length Polymorphisms of 16S RRNA Genes in the Differentiation of Fast-growing Mycobacterial Species

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 1994 Feb 1

PMID 7907567

Citations 23

Authors

P Domenech

M C Menendez

M J Garcia

Affiliations

Soon will be listed here.

Abstract

DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA-DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast-growing mycobacteria.

Citing Articles

Methods of phenotypic identification of non-tuberculous mycobacteria.

Bhalla G, Sarao M, Kalra D, Bandyopadhyay K, John A Pract Lab Med. 2018; 12:e00107.

PMID: 30094310 PMC: 6074605. DOI: 10.1016/j.plabm.2018.e00107.

Molecular analysis of clinical isolates previously diagnosed as Mycobacterium intracellulare reveals incidental findings of "Mycobacterium indicus pranii" genotypes in human lung infection.

Kim S, Park H, Jeong B, Jeon K, Huh H, Ki C BMC Infect Dis. 2015; 15:406.

PMID: 26423052 PMC: 4589961. DOI: 10.1186/s12879-015-1140-4.

The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.

Leao S, Matsumoto C, Carneiro A, Ramos R, Lourenco Nogueira C, Lima Jr J PLoS One. 2013; 8(4):e60746.

PMID: 23565273 PMC: 3614916. DOI: 10.1371/journal.pone.0060746.

Combined rpoB duplex PCR and hsp65 PCR restriction fragment length polymorphism with capillary electrophoresis as an effective algorithm for identification of mycobacterial species from clinical isolates.

Huang C, Chen J, Hu S, Chiou C, Huang W, Hsu J BMC Microbiol. 2012; 12:137.

PMID: 22769981 PMC: 3511182. DOI: 10.1186/1471-2180-12-137.

Physiology of mycobacteria.

Cook G, Berney M, Gebhard S, Heinemann M, Cox R, Danilchanka O Adv Microb Physiol. 2009; 55:81-182, 318-9.

PMID: 19573696 PMC: 3728839. DOI: 10.1016/S0065-2911(09)05502-7.