Mapping Translocation Breakpoints by Orthogonal Field Agarose-gel Electrophoresis

Overview

Journal Curr Genet

Specialty Genetics

Date 1996 Feb 1

PMID 8595678

Citations 4

Authors

M L Smith

N L Glass

Affiliations

Soon will be listed here.

Abstract

Orthogonal field agarose-gel electrophoresis (OFAGE) of chromosomes from translocation-bearing and normal Neurospora crassa strains was utilized, first, to recover cosmids from a translocated region, and second, to map translocation breakpoints. Surprisingly, the right breakpoints in two independently derived, interstitial translocations, T(II-->III) AR18 and T(II-->VI)P2869, are within about 5.6 kbp of each other suggesting that this region of linkage group (LG) II may be fragile or otherwise subject to chromosome breakage. Mapping translocation breakpoints through OFAGE, or other similar methods, should allow for DNA sequencing across breakpoints that are not associated with mutant phenotypes or that are not within walking distance of cloned markers.

Citing Articles

Titration of repeat-induced point mutation (RIP) by chromosome segment duplications in Neurospora crassa.

Singh P, Kasbekar D Genetica. 2007; 134(3):267-75.

PMID: 18046508 DOI: 10.1007/s10709-007-9234-6.

Chromosome segment duplications in Neurospora crassa and their effects on repeat-induced point mutation and meiotic silencing by unpaired DNA.

Vyas M, Ravindran C, Kasbekar D Genetics. 2005; 172(3):1511-9.

PMID: 16361235 PMC: 1456276. DOI: 10.1534/genetics.105.050468.

Vegetative incompatibility in the het-6 region of Neurospora crassa is mediated by two linked genes.

Smith M, Micali O, Hubbard S, Jacobson D, Glass N Genetics. 2000; 155(3):1095-104.

PMID: 10880472 PMC: 1461168. DOI: 10.1093/genetics/155.3.1095.

Escape from het-6 incompatibility in Neurospora crassa partial diploids involves preferential deletion within the ectopic segment.

Smith M, Yang C, Metzenberg R, Glass N Genetics. 1996; 144(2):523-31.

PMID: 8889517 PMC: 1207547. DOI: 10.1093/genetics/144.2.523.

References

Toth G, Jurka J . Repetitive DNA in and around translocation breakpoints of the Philadelphia chromosome. Gene. 1994; 140(2):285-8. DOI: 10.1016/0378-1119(94)90559-2. View

Vollmer S, Yanofsky C . Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A. 1986; 83(13):4869-73. PMC: 323844. DOI: 10.1073/pnas.83.13.4869. View

Tsubota S, Rosenberg D, Szostak H, Rubin D, Schedl P . The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: evidence for a transposon-induced rearrangement. Genetics. 1989; 122(4):881-90. PMC: 1203762. DOI: 10.1093/genetics/122.4.881. View

Perkins D, Kinsey J, Asch D, Frederick G . Chromosome rearrangements recovered following transformation of Neurospora crassa. Genetics. 1993; 134(3):729-36. PMC: 1205511. DOI: 10.1093/genetics/134.3.729. View

Metzenberg R, Stevens J, Selker E . Identification and chromosomal distribution of 5S rRNA genes in Neurospora crassa. Proc Natl Acad Sci U S A. 1985; 82(7):2067-71. PMC: 397493. DOI: 10.1073/pnas.82.7.2067. View

Paluh J, Plamann M, Kruger D, Barthelmess I, Yanofsky C, Perkins D . Determination of the inactivating alterations in two mutant alleles of the Neurospora crassa cross-pathway control gene cpc-1. Genetics. 1990; 124(3):599-606. PMC: 1203953. DOI: 10.1093/genetics/124.3.599. View

Antonarakis S . Human chromosome 21: genome mapping and exploration, circa 1993. Trends Genet. 1993; 9(4):142-8. DOI: 10.1016/0168-9525(93)90210-9. View

Chen S, Chen Z, Font M, dAuriol L, Larsen C, Berger R . Structural alterations of the BCR and ABL genes in Ph1 positive acute leukemias with rearrangements in the BCR gene first intron: further evidence implicating Alu sequences in the chromosome translocation. Nucleic Acids Res. 1989; 17(19):7631-42. PMC: 334872. DOI: 10.1093/nar/17.19.7631. View

Kang S, Metzenberg R . Molecular analysis of nuc-1+, a gene controlling phosphorus acquisition in Neurospora crassa. Mol Cell Biol. 1990; 10(11):5839-48. PMC: 361368. DOI: 10.1128/mcb.10.11.5839-5848.1990. View

10.

Cirera S, Martin-Campos J, Segarra C, Aguade M . Molecular characterization of the breakpoints of an inversion fixed between Drosophila melanogaster and D. subobscura. Genetics. 1995; 139(1):321-6. PMC: 1206328. DOI: 10.1093/genetics/139.1.321. View

11.

Doring H, Garber R, Tillmann E . Double Ds elements are involved in specific chromosome breakage. Mol Gen Genet. 1989; 219(1-2):299-305. DOI: 10.1007/BF00261191. View

12.

Kinsey J, Helber J . Isolation of a transposable element from Neurospora crassa. Proc Natl Acad Sci U S A. 1989; 86(6):1929-33. PMC: 286818. DOI: 10.1073/pnas.86.6.1929. View

13.

Perkins D, Radford A, Newmeyer D, Bjorkman M . Chromosomal loci of Neurospora crassa. Microbiol Rev. 1982; 46(4):426-570. PMC: 281555. DOI: 10.1128/mr.46.4.426-570.1982. View

14.

Engels W, Preston C . Formation of chromosome rearrangements by P factors in Drosophila. Genetics. 1984; 107(4):657-78. PMC: 1202383. DOI: 10.1093/genetics/107.4.657. View

15.

Schmidhauser T, Lauter F, Russo V, Yanofsky C . Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa. Mol Cell Biol. 1990; 10(10):5064-70. PMC: 361171. DOI: 10.1128/mcb.10.10.5064-5070.1990. View

16.

Wesley C, Eanes W . Isolation and analysis of the breakpoint sequences of chromosome inversion In(3L)Payne in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1994; 91(8):3132-6. PMC: 43529. DOI: 10.1073/pnas.91.8.3132. View

17.

Krumlauf R, Marzluf G . Genome organization and characterization of the repetitive and inverted repeat DNA sequences in Neurospora crassa. J Biol Chem. 1980; 255(3):1138-45. View

18.

Orbach M, Vollrath D, Davis R, Yanofsky C . An electrophoretic karyotype of Neurospora crassa. Mol Cell Biol. 1988; 8(4):1469-73. PMC: 363304. DOI: 10.1128/mcb.8.4.1469-1473.1988. View

19.

Longo E, Vezinhet F . Chromosomal rearrangements during vegetative growth of a wild strain of Saccharomyces cerevisiae. Appl Environ Microbiol. 1993; 59(1):322-6. PMC: 202098. DOI: 10.1128/aem.59.1.322-326.1993. View

20.

Glass N, Smith M . Structure and function of a mating-type gene from the homothallic species Neurospora africana. Mol Gen Genet. 1994; 244(4):401-9. DOI: 10.1007/BF00286692. View