An Electrophoretic Karyotype of Neurospora Crassa

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1988 Apr 1

PMID 2967910

Citations 90

Authors

M J Orbach

D Vollrath

R W Davis

C Yanofsky

Affiliations

Soon will be listed here.

Abstract

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

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References

Schwartz D, Cantor C . Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984; 37(1):67-75. DOI: 10.1016/0092-8674(84)90301-5. View

Orbach M, Porro E, Yanofsky C . Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol. 1986; 6(7):2452-61. PMC: 367799. DOI: 10.1128/mcb.6.7.2452-2461.1986. View

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

Buxton F, Radford A . Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli. Mol Gen Genet. 1983; 190(3):403-5. DOI: 10.1007/BF00331067. View

Carle G, Olson M . An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985; 82(11):3756-60. PMC: 397866. DOI: 10.1073/pnas.82.11.3756. View

Watson J, Crick F . Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953; 171(4356):737-8. DOI: 10.1038/171737a0. View

Russell P, Wagner S, Rodland K, Feinbaum R, Russell J, Bret-Harte M . Organization of the ribosomal ribonucleic acid genes in various wild-type strains and wild-collected strains of Neurospora. Mol Gen Genet. 1984; 196(2):275-82. DOI: 10.1007/BF00328060. View

Vollmer S, Yanofsky C . Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A. 1986; 83(13):4869-73. PMC: 323844. DOI: 10.1073/pnas.83.13.4869. View

MORTIMER R, Schild D . Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol Rev. 1985; 49(3):181-213. PMC: 373033. DOI: 10.1128/mr.49.3.181-213.1985. View

10.

Krumlauf R, Marzluf G . Characterization of the sequence complexity and organization of the Neurospora crassa genome. Biochemistry. 1979; 18(17):3705-13. DOI: 10.1021/bi00584a011. View

11.

Smith C, Matsumoto T, Niwa O, Klco S, Fan J, Yanagida M . An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Res. 1987; 15(11):4481-9. PMC: 340875. DOI: 10.1093/nar/15.11.4481. View

12.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

13.

Chu G, Vollrath D, Davis R . Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986; 234(4783):1582-5. DOI: 10.1126/science.3538420. View

14.

Legerton T, Yanofsky C . Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa. Gene. 1985; 39(2-3):129-40. DOI: 10.1016/0378-1119(85)90306-3. View

15.

Vollrath D, Davis R . Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res. 1987; 15(19):7865-76. PMC: 306313. DOI: 10.1093/nar/15.19.7865. View

16.

Perkins D, Radford A, Newmeyer D, Bjorkman M . Chromosomal loci of Neurospora crassa. Microbiol Rev. 1982; 46(4):426-570. PMC: 281555. DOI: 10.1128/mr.46.4.426-570.1982. View

17.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

18.

Schechtman M, Yanofsky C . Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli. J Mol Appl Genet. 1983; 2(1):83-99. View

19.

Barratt R, Newmeyer D, Perkins D, GARNJOBST L . Map construction in Neurospora crassa. Adv Genet. 1954; 6:1-93. DOI: 10.1016/s0065-2660(08)60127-3. View

20.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View