Cloning, Sequence, and Photoregulation of Al-1, a Carotenoid Biosynthetic Gene of Neurospora Crassa

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1990 Oct 1

PMID 2144609

Citations 49

Authors

T J Schmidhauser

F R Lauter

V E Russo

C Yanofsky

Affiliations

Soon will be listed here.

Abstract

Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene of N. crassa encoding the carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis of its structure and regulation. The gene encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed that the level of al-1 mRNA increased over 70-fold in photoinduced mycelia. Transcription run-on studies indicated that the al-1 gene was regulated at the level of initiation of transcription in response to photoinduction. The photoinduced increase of al-1 mRNA levels was not observed in two Neurospora mutants defective in all physiological photoresponses. Analysis of cosmid containing al-1 and of a translocation strain with a breakpoint within al-1 indicated that al-1 transcription proceeds towards the centromere of linkage group I of N. crassa.

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References

Orbach M, Porro E, Yanofsky C . Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol. 1986; 6(7):2452-61. PMC: 367799. DOI: 10.1128/mcb.6.7.2452-2461.1986. View

Simpson K . Relative value of carotenoids as precursors of vitamin A. Proc Nutr Soc. 1983; 42(1):7-17. DOI: 10.1079/pns19830003. View

Goldie A, Subden R . The neutral carotenoids of wild-type and mutant strains of Neurospora crassa. Biochem Genet. 1973; 10(3):275-84. DOI: 10.1007/BF00485705. View

Innocenti F, Pohl U, Russo V . Photoinduction of protoperithecia in Neurospora crassa by blue light. Photochem Photobiol. 1983; 37(1):49-51. DOI: 10.1111/j.1751-1097.1983.tb04432.x. View

Sommer T, Chambers J, Eberle J, Lauter F, Russo V . Fast light-regulated genes of Neurospora crassa. Nucleic Acids Res. 1989; 17(14):5713-23. PMC: 318191. DOI: 10.1093/nar/17.14.5713. View

Harding R, Turner R . Photoregulation of the Carotenoid Biosynthetic Pathway in Albino and White Collar Mutants of Neurospora crassa. Plant Physiol. 1981; 68(3):745-9. PMC: 425974. DOI: 10.1104/pp.68.3.745. View

Degli-Innocenti F, Russo V . Isolation of new white collar mutants of Neurospora crassa and studies on their behavior in the blue light-induced formation of protoperithecia. J Bacteriol. 1984; 159(2):757-61. PMC: 215710. DOI: 10.1128/jb.159.2.757-761.1984. View

Vollmer S, Yanofsky C . Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A. 1986; 83(13):4869-73. PMC: 323844. DOI: 10.1073/pnas.83.13.4869. View

Boll W, Fujisawa J, Niemi J, Weissmann C . A new approach to high sensitivity differential hybridization. Gene. 1986; 50(1-3):41-53. DOI: 10.1016/0378-1119(86)90308-2. View

10.

Bendich A, Olson J . Biological actions of carotenoids. FASEB J. 1989; 3(8):1927-32. View

11.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

12.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

13.

Legerton T, Yanofsky C . Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa. Gene. 1985; 39(2-3):129-40. DOI: 10.1016/0378-1119(85)90306-3. View

14.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

15.

Willmitzer L, Wagner K . The isolation of nuclei from tissue-cultured plant cells. Exp Cell Res. 1981; 135(1):69-77. DOI: 10.1016/0014-4827(81)90300-1. View

16.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

17.

Thomas S, Sargent M, Tuveson R . Inactivation of normal and mutant Neurospora crassa conidia by visible light and near-UV: role of 1O2, carotenoid composition and sensitizer location. Photochem Photobiol. 1981; 33(3):349-54. DOI: 10.1111/j.1751-1097.1981.tb05428.x. View

18.

Perkins D, Radford A, Newmeyer D, Bjorkman M . Chromosomal loci of Neurospora crassa. Microbiol Rev. 1982; 46(4):426-570. PMC: 281555. DOI: 10.1128/mr.46.4.426-570.1982. View

19.

Kinsey J, Rambosek J . Transformation of Neurospora crassa with the cloned am (glutamate dehydrogenase) gene. Mol Cell Biol. 1984; 4(1):117-22. PMC: 368665. DOI: 10.1128/mcb.4.1.117-122.1984. View

20.

Armstrong G, Alberti M, Leach F, Hearst J . Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol Gen Genet. 1989; 216(2-3):254-68. DOI: 10.1007/BF00334364. View