Infrequent Ras Mutation in Human Stomach Cancers

Overview

Journal Jpn J Cancer Res

Specialty Oncology

Date 1993 Feb 1

PMID 8463133

Citations 6

Authors

M Koshiba

O Ogawa

T Habuchi

S Hamazaki

T Shimada

R Takahashi

T Sugiyama

Affiliations

Soon will be listed here.

Abstract

Mutations of ras oncogenes in 37 human stomach cancers and 13 adenomas were investigated with regard to the histological phenotypes using polymerase chain reaction (PCR), allele-specific oligonucleotide hybridization and/or direct sequencing of the PCR products. The ras mutation was found only in one case (2.7%), the histology of which was poorly differentiated adenocarcinoma. We found no mutation in stomach adenomas. The mutation consisted of a guanine-to-adenine transition in the first base of codon 13 of c-Ki-ras which replaced wild-type glycine with serine, indicating that a putative glycine-to-aspartic acid change is not necessarily the critical event for c-Ki-ras gene activation in codon 13. These results further confirm the infrequency of ras mutation in stomach tumors and also suggest that ras mutations are not specific to the differentiated type of stomach cancer.

Citing Articles

A microRNA contribution to aberrant Ras activation in gastric cancer.

Lam E, Wang X, Shin V, Zhang S, Morrison H, Sun J Am J Transl Res. 2011; 3(2):209-18.

PMID: 21416062 PMC: 3056566.

Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea.

Furihata C, Tatematsu M, Saito M, Ishida S, Nakanishi H, Inada K Jpn J Cancer Res. 1997; 88(4):363-8.

PMID: 9197527 PMC: 5921434. DOI: 10.1111/j.1349-7006.1997.tb00390.x.

Detection of c-Ki-ras oncogene mutation in gastric adenomas with formalin-fixed, paraffin-embedded biopsy materials.

Tsuchiya C, Ohshima S, Takahama M J Gastroenterol. 1997; 32(1):28-33.

PMID: 9058292 DOI: 10.1007/BF01213293.

Frequent K-ras mutations in small bowel adenocarcinomas.

Sutter T, Arber N, Moss S, Findling R, Neugut A, Weinstein I Dig Dis Sci. 1996; 41(1):115-8.

PMID: 8565742 DOI: 10.1007/BF02208591.

Gastric adenoma-carcinoma sequence with special reference to p53 and Ki-ras gene alterations.

Sakurai S, Sano T, Maeshima A, Kashiwabara K, Oyama T, Fukuda T Virchows Arch. 1995; 427(2):119-24.

PMID: 7582240 DOI: 10.1007/BF00196515.

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