Increased Expression of Ras Genes in Non-Hodgkin's Lymphomas is Not Associated with Oncogenic Activation of Those Genes by Point Mutation

Overview

Journal Jpn J Cancer Res

Specialty Oncology

Date 1989 Aug 1

PMID 2511175

Citations 3

Authors

K Hamatani

Y Nagata

M Abe

K Abe

H Toki

S Ikeda

E Nakayama

H Shiku

Affiliations

Soon will be listed here.

Abstract

Twenty-three cases of non-Hodgkin's lymphoma (NHL) were analyzed for expression of ras genes by in situ hybridization utilizing biotinylated DNA probes. Increased expression of Ki-ras, Ha-ras and N-ras genes was observed in 12 cases, 6 cases and 1 case of NHL, respectively. Genomic DNA extracted from these 23 cases of NHL was region-specifically amplified by means of polymerase chain reaction to examine the presence of point mutations at the 12th, 13th and 61st codons of Ki-, Ha- and N-ras genes. Dot hybridization assays with appropriate oligonucleotide probes showed no evidence of point mutation in any case of NHL examined. These results indicate that increased expression of ras genes in NHL is not associated with ras gene activation by point mutation.

Citing Articles

Infrequent ras mutation in human stomach cancers.

Koshiba M, Ogawa O, Habuchi T, Hamazaki S, Shimada T, Takahashi R Jpn J Cancer Res. 1993; 84(2):163-7.

PMID: 8463133 PMC: 5919135. DOI: 10.1111/j.1349-7006.1993.tb02850.x.

Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japanese.

Nagata Y, Abe M, Motoshima K, Nakayama E, Shiku H Jpn J Cancer Res. 1990; 81(2):135-40.

PMID: 2110130 PMC: 5963896. DOI: 10.1111/j.1349-7006.1990.tb02539.x.

Point mutations of c-ras genes in human bladder cancer and kidney cancer.

Nagata Y, Abe M, Kobayashi K, Saiki S, Kotake T, Yoshikawa K Jpn J Cancer Res. 1990; 81(1):22-7.

PMID: 2108944 PMC: 5917946. DOI: 10.1111/j.1349-7006.1990.tb02502.x.

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