The Human C-Kirsten Ras Gene is Activated by a Novel Mutation in Codon 13 in the Breast Carcinoma Cell Line MDA-MB231

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Aug 11

PMID 3627975

Citations 52

Authors

S C Kozma

M E Bogaard

K Buser

S M Saurer

J L Bos

B Groner

N E Hynes

Affiliations

Soon will be listed here.

Abstract

We have detected amplified human Ki-ras sequences in tumorigenic NIH 3T3 cells transfected with genomic DNA from the human breast carcinoma cell line MDA-MB231. Hybridization of synthetic oligonucleotides specific for human Ki-ras sequences showed a mutation at codon 13. The polymerase chain reaction with Ki-ras specific amplimers revealed a guanosine to adenosine transition at the second position of codon 13, resulting in a substitution of glycine by aspartic acid. The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line.

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