K-sam, an Amplified Gene in Stomach Cancer, is a Member of the Heparin-binding Growth Factor Receptor Genes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Aug 1

PMID 2377625

Citations 79

Authors

Y Hattori

H Odagiri

H Nakatani

K Miyagawa

K Naito

H Sakamoto

O Katoh

T Yoshida

T Sugimura

M Terada

Affiliations

Soon will be listed here.

Abstract

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

Citing Articles

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